Cell Signaling Technology

Product Pathways - Protein Stability

USP15 (D1K6S) Rabbit mAb #66310

DUBs   Unph-2   Unph4   USP15  

No. Size Price
66310S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
66310 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 120 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Dog, Pig,

Specificity / Sensitivity

USP15 (D1K6S) Rabbit mAb recognizes endogenous levels of total USP15 protein. This antibody does not cross-react with either USP4 or USP11 proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human USP15 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using USP15 (D1K6S) Rabbit mAb.

IP

IP

Immunoprecipitation of USP15 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is USP15 (D1K6S) Rabbit mAb. Western blot analysis was performed using USP15 (D1K6S) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human USP4 protein (hUSP4-Myc/DDK; +), Myc/DDK-tagged full-length USP11 protein (hUSP11-Myc/DDK; +), and Myc/DDK-tagged full-length human USP15 protein (hUSP15-Myc/DDK; +), using USP15 (D1K6S) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).

Background

Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs), respectively (1,2). DUBs are categorized into five subfamilies based on catalytic domain structure: USP, UCH, OTU, MJD, and JAMM. Ubiquitin carboxyl-terminal hydrolase 15 (USP15) is a USP subfamily deubiquitinating enzyme with similar domain structure to the paralogous DUBs, USP4, and USP11. The USP15 gene is amplified in glioblastoma and other solid tumors and its high expression correlates with a poor prognosis (3,4). Research studies demonstrate that USP15 is a positive regulator of oncogenic TGF-β signaling and that USP15 deubiquitinates monoubiquitinated R-SMADs to enhance target gene promoter binding (5). USP15 also promotes oncogenic TGF-β signaling by opposing SMURF2-mediated ubiquitination of the type I TGF-β receptor, which facilitates receptor stabilization (3,4). USP15 contributes to oncogenesis by negatively regulating T cell-mediated antitumor responses through the deubiquitination and stabilization of the E3 ubiquitin ligase MDM2. This observation supports USP15 as a potential target for cancer therapeutics (6).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Zhang, L. et al. (2013) Mol Cell 51, 559-72.
  4. Eichhorn, P.J. et al. (2012) Nat Med 18, 429-35.
  5. Inui, M. et al. (2011) Nat Cell Biol 13, 1368-75.
  6. Zou, Q. et al. (2014) Nat Immunol 15, 562-70.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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