Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Yes (D9P3E) Rabbit mAb #65890

No. Size Price
65890S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
65890 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 60 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Yes (D9P3E) Rabbit mAb recognizes endogenous levels of total Yes protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Yes protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Yes (D9P3E) Rabbit mAb.

IP

IP

Immunoprecipitation of Yes protein from U118 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Yes (D9P3E) Rabbit mAb. Western blot analysis was performed using Yes (D9P3E) Rabbit mAb.

Background

The cellular oncogene c-Yes and its viral homologue v-Yes (the transforming gene of Yamaguchi 73 and Esh avian sarcoma viruses) encode a 60 kDa, cytoplasmic, membrane-associated, protein-tyrosine kinase (1). Yes belongs to the Src kinase family and is ubiquitously expressed in many tissues and cells. Like other Src family members, Yes contains several conserved functional domains such as an N-terminal myristoylation sequence for membrane targeting, SH2 and SH3 domains, a kinase domain, and a C-terminal non-catalytic domain (2). Although several lines of evidence support redundancy in signaling between Yes and other Src family kinases, there is also a growing body of evidence indicating specificity in Yes signaling (2). Yes is activated downstream of a multitude of cell surface receptors, including receptor tyrosine kinases, G protein-coupled receptors, and cytokine receptors (3). In addition, both Yes and Src kinases are activated during the cell cycle transition from G2 to M phase (3). Investigators have found that dysfunction of Yes is associated with the development of various cancers (4).

  1. Pena, S.V. et al. (1995) Gastroenterology 108, 117-24.
  2. Summy, J.M. et al. (2003) Front Biosci 8, s185-205.
  3. Summy, J.M. et al. (2003) J Cell Sci 116, 2585-98.
  4. Rungsipipat, A. et al. (1999) Res Vet Sci 66, 205-10.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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