Cell Signaling Technology

Product Pathways - Protein Stability

UBR5 (D6O8Z) Rabbit mAb #65344

E3 ubquitin ligases   EDD   EDD1   HECT E3 ligases   HYD   UBR5  

No. Size Price
65344S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
65344 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 300 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Dog, Pig,

Specificity / Sensitivity

UBR5 (D6O8Z) Rabbit mAb recognizes endogenous levels of total UBR5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBR5 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UBR5 (D6O8Z) Rabbit mAb.

IP

IP

Immunoprecipitation of UBR5 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is UBR5 (D6O8Z) Rabbit mAb. Western blot analysis was performed using UBR5 (D6O8Z) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with siRNA targeting human UBR5 (+), using UBR5 (D6O8Z) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Background

Human E3 identified by differential display (UBR5/EDD) is a HECT domain-containing ubiquitin E3 ligase of the N-end rule pathway that promotes the ubiquitination and proteasomal degradation of proteins harboring N-degrons (1-3). UBR5 represents an ortholog of HYD, the Drosophila hyperplastic discs tumor suppressor gene product but has been found to be overexpressed in breast and ovarian cancers, suggesting a possible role in promoting tumor development (4,5). Research studies have demonstrated that UBR5 is functional within the nucleus as it participates in DNA damage signaling by controlling the activities of Chk2, TopBP1, and RNF168 (6-9). Recently, UBR5 was shown to play a novel role in immune cell function by regulating RORγt stability and IL-17 production by Th17 cells (10).

  1. Sriram, S.M. et al. (2011) Nat Rev Mol Cell Biol 12, 735-47.
  2. Callaghan, M.J. et al. (1998) Oncogene 17, 3479-91.
  3. Tasaki, T. et al. (2005) Mol Cell Biol 25, 7120-36.
  4. Clancy, J.L. et al. (2003) Oncogene 22, 5070-81.
  5. O'Brien, P.M. et al. (2008) Br J Cancer 98, 1085-93.
  6. Munoz, M.A. et al. (2007) Cell Cycle 6, 3070-7.
  7. Henderson, M.J. et al. (2006) J Biol Chem 281, 39990-40000.
  8. Honda, Y. et al. (2002) J Biol Chem 277, 3599-605.
  9. Gudjonsson, T. et al. (2012) Cell 150, 697-709.
  10. Rutz, S. et al. (2015) Nature 518, 417-21.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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