Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) #64988

B7-H1   B7H1   CD274   PD-L1   PDCD Ligand 1   PDCD1LG1   Programmed cell death 1 ligand 1  

No. Size Price
64988S 100 µl ( 200 sections ) ¥3,480.00 现货查询 购买询价 防伪查询
64988 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IHC-P 1:200 Mouse, Endogenous 40-50 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific) recognizes endogenous levels of total mouse PD-L1 protein. Non-specific staining of keratin has been observed.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly215 of mouse PD-L1 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal mouse thymus using PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded normal mouse lung using PD-L1 (D5V3B) Rabbit mAb (Mouse Specific; IHC Specific).

Background

Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9).

  1. Dong, H. et al. (1999) Nat Med 5, 1365-9.
  2. Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
  3. Liang, S.C. et al. (2003) Eur J Immunol 33, 2706-16.
  4. Dong, H. et al. (2002) Nat Med 8, 793-800.
  5. Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5.
  6. Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64.
  7. Taube, J.M. et al. (2012) Sci Transl Med 4, 127ra37.
  8. Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41.
  9. Chen, B.J. et al. (2013) Clin Cancer Res 19, 3462-73.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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