Cell Signaling Technology

Product Pathways - Tag Antibodies

Myc-Tag (71D10) Rabbit mAb (PE Conjugate) #64025

No. Size Price
64025S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
64025 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 All Species Expected, Transfected Only Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

Myc-Tag (71D10) Rabbit mAb (PE Conjugate) detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Myc-Tag (71D10) Rabbit mAb #2278.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of COS-7 cells, untransfected (blue) or transfected with a Myc-tagged fusion protein, using Myc-Tag (71D10) Rabbit mAb (PE Conjugate) (green).

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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