Cell Signaling Technology

Product Pathways - Protein Stability

Napsin A (D5P6G) XP® Rabbit mAb #62434

No. Size Price
62434S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
62434T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
62434 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 31 Rabbit IgG
IHC-P 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Napsin A (D5P6G) XP® Rabbit mAb recognizes endogenous levels of total napsin A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human napsin A protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Napsin A (D5P6G) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma (left) or squamous cell lung carcinoma (right) using Napsin A (D5P6G) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded H3255 (left) or 293 (right) cell pellets using Napsin A (D5P6G) XP® Rabbit mAb.

Background

Napsin A is an aspartic proteinase that is expressed in normal lung and kidney (1). In the lung, napsin A is expressed by type II pneumocytes and alveolar macrophages, where it plays a role in processing surfactant protein B (2). Napsin A is expressed in lung adenocarcinomas, where it can be used to identify primary and metastatic lesions with greater sensitivity compared to TTF-1 (3,4). Napsin A expression has also been described in other types of cancer, such as kidney and thyroid cancer (5).

  1. Tatnell, P.J. et al. (1998) FEBS Lett 441, 43-8.
  2. Schauer-Vukasinovic, V. et al. (1999) FEBS Lett 462, 135-9.
  3. Jagirdar, J. (2008) Arch Pathol Lab Med 132, 384-96.
  4. Turner, B.M. et al. (2012) Arch Pathol Lab Med 136, 163-71.
  5. Bishop, J.A. et al. (2010) Hum Pathol 41, 20-5.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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