Cell Signaling Technology

Product Pathways - Metabolism

NAF-1/CISD2 (D4X4E) Rabbit mAb #60758

No. Size Price
60758S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
60758 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 15 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

NAF-1/CISD2 (D4X4E) Rabbit mAb recognizes endogenous levels of total NAF-1/CISD2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu70 of human NAF-1/CISD2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NAF-1/CISD2 (D4X4E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Nutrient-deprivation autophagy factor-1 (NAF-1) (also known as CISD2, CDGSH iron-sulfur domain-containing protein 2) is a member of NEET family of 2Fe-2S proteins, characterized by a unique CDGSH sequence at their Fe-S-cluster-binding domain (1). NAF-1/CISD2 is a multifunctional protein. In addition to its role in iron and ROS homeostasis, it has been shown to play a role in autophagy, neurodegenerative diseases, and aging (2-7). Enhanced expression of NAF-1/CISD2 is associated with many types of cancer. Silencing of NAF-1/CISD2 expression in cancer cells significantly inhibited proliferation and tumorigenicity; while overexpression of NAF-1/CISD2 significantly enhanced proliferation (2, 8, 9).

  1. Tamir, S. et al. (2015) Biochim Biophys Acta 1853, 1294-315.
  2. Chang, N.C. et al. (2012) Autophagy 8, 856-7.
  3. Lipper, C.H. et al. (2015) PLoS One 10, e0139699.
  4. Du, X. et al. (2015) Cell Biol Int 39, 816-23.
  5. Chen, Y.F. et al. (2009) Genes Dev 23, 1183-94.
  6. Chen, Y.F. et al. (2010) Ann N Y Acad Sci 1201, 58-64.
  7. Yang, L. et al. (2016) Oncotarget 7, 22720-32.
  8. Holt, S.H. et al. (2016) J Cell Sci 129, 155-65.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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