Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Desmoplakin (Ser165/166) Antibody #5885

No. Size Price
5885S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
5885 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 250 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Desmoplakin (Ser165/166) Antibody recognizes endogenous levels of desmoplakin protein only when phosphorylated at Ser165/166.

Phospho-Desmoplakin (Ser165/166)抗体识别165/166位丝氨酸磷酸化后内源性desmoplakin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser165/166 of human desmoplakin protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 and Calu-3 cells, untreated or treated with calf intestinal phosphatase (CIP) and λ phosphatase, using Phospho-Desmoplakin (Ser165/166) Antibody.未处理或经过牛小肠碱性磷酸酶(CIP)和λ磷酸酶处理的A-431和Calu-3细胞提取物进行western blot分析,使用Phospho-Desmoplakin (Ser165/166)抗体进行实验。


Desmosomes are a class of intracellular junction that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium (1). They derive their characteristic strength from the protein Desmoplakin, which acts as a tether by binding the cytoplasmic component of the Desmosome at it’s N-terminus (2) while its C-terminus is anchored to the intermediate-filament cytoskeleton (3). This association mitigates the impact of mechanical forces on the desmosome by distributing them throughout the cytoskeleton and tissue (4). Desmoplakin is essential for normal desmosomal adhesion (5); defects can result in pathologies that include cardiomyopathy (6), keratoderma (7), or the skin blistering disease Epidermolysis bullosa (8).


Phospho-Desmoplakin (Ser165/166) Antibody is directed against a site that was identified at Cell Signaling Technology (CST) using PTMScan®, our LC-MS/MS platform for modification site discovery. Please visit PhosphoSitePlus®, a modification site knowledgebase developed and maintained by CST, at www.phosphosite.org for more information.

Cell Signaling Technology (CST) 使用PTMScan®识别的位点以设计Phospho-Desmoplakin (Ser165/166)抗体,本公司LC-MS/MS平台用于发现修饰位点。请访问PhosphoSitePlus®,一个由CST设计的修饰位点数据库,更多信息请访问www.phosphosite.org。

  1. Stokes, D.L. (2007) Curr Opin Cell Biol 19, 565-71.
  2. Kowalczyk, A.P. et al. (1997) J Cell Biol 139, 773-84.
  3. Meng, J.J. et al. (1997) J Biol Chem 272, 21495-503.
  4. Green, K.J. and Simpson, C.L. (2007) J Invest Dermatol 127, 2499-515.
  5. Vasioukhin, V. et al. (2001) Nat Cell Biol 3, 1076-85.
  6. Rampazzo, A. et al. (2002) Am J Hum Genet 71, 1200-6.
  7. Armstrong, D.K. et al. (1999) Hum Mol Genet 8, 143-8.
  8. Jonkman, M.F. et al. (2005) Am J Hum Genet 77, 653-60.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :