Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Axl (Tyr702) (D12B2) Rabbit mAb #5724

No. Size Price
5724S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
5724 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 138 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-Axl (Tyr702) (D12B2) Rabbit mAb detects endogenous levels of Axl only when phosphorylated at Tyr702. This antibody may also cross-react with other overexpressed, related tyrosine-phosphorylated tyrosine kinases, such as EGFR. 磷酸化的Axl (Tyr702)的兔源单克隆抗体仅在Tyr702被磷酸化后才能检测到Axl的存在。本抗体也能与其它过表达的相关磷酸化酪氨酸激酶(如EGFR)交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr702 of human Axl protein. 单克隆抗体通过免疫动物得到,该合成磷酸化多肽是根据人的Axl蛋白Tyr702附近的氨基酸序列合成的。

Western Blotting

Western Blotting

Western blot analysis of extracts from NCI-H1299 cells, untreated or Gas6-treated (100 ng/ml for 10 minutes), using Phospho-Axl (Tyr702) (D12B2) Rabbit mAb (upper) or Axl (C44G1) Rabbit mAb #4566 (lower). 用抗磷酸化的Axl (Tyr702)的兔源单克隆抗体(上)或抗Axl (C44G1) 的兔源单克隆抗体(下)对经过或未经Gas6 (100 ng/ml , 10分钟)处理的NCI-H1299细胞的提取物进行免疫印迹检测。


Axl, Sky and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).

Axl、 Sky和 Mer是 受体酪氨酸激酶 (RTK) 家族的三个成员,它们有共同的胞内酪氨酸激酶区域和与胞外粘附分子相似的胞外区。这些受体酪氨酸激酶与维生素K依赖性的生长停滞特异性6蛋白(Gas6)结合,Gas6蛋白与S抗凝血因子蛋白结构上相关(1)。与受体结合后,Gas6能够激活PI3K激酶和它的下游靶分子Akt、 S6K和 NF-κB (2,3)。大量证据表明Gas6/Axl信号通路与正常细胞和癌细胞的生长和存活相关(4)。

Phosphorylation of Axl Receptor on Tyr702 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery (5). Phosphorylation of Axl on Tyr702 was also reported in select carcinoma cell lines (6).

Axl的Tyr1702位点磷酸化是Cell Signaling Technology (CST)公司利用LC-MS/MS平台PhosphoScan®技术在寻找磷酸化位点过程中发现的(5)。Axl的Tyr702位点磷酸化在某些肿瘤细胞系中存在(6)。

  1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-5.
  2. Demarchi, F. et al. (2001) J Biol Chem 276, 31738-44.
  3. Lee, W.P. et al. (2002) Oncogene 21, 329-36.
  4. Bellosta, P. et al. (1997) Oncogene 15, 2387-97.
  5. Rikova, K. et al. (2007) Cell 131, 1190-203.
  6. Guo, A. et al. (2008) Proc Natl Acad Sci USA 105, 692-7.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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