Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

SH3BP4 (D3D9I) Rabbit mAb #55935

BOG25   EH-binding protein 10   EHB10   SH3 domain-binding protein 4   SH3-domain binding protein 4   SH3B4   SH3BP4   transferrin receptor trafficking protein   Transferrin receptor-trafficking protein   TTP  

No. Size Price
55935S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
55935 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 110 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

SH3BP4 (D3D9I) Rabbit mAb recognizes endogenous levels of total SH3BP4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val222 of human SH3BP4 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SH3BP4 (D3D9I) Rabbit mAb.

IP

IP

Immunoprecipitation of SH3BP4 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SH3BP4 (D3D9I) Rabbit mAb. Western blot analysis was performed using SH3BP4 (D3D9I) Rabbit mAb.

Background

The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by enviromental factors such as energy levels, growth factors, and amino acids (3, 4). The GTPases RagA, RagB, RagC, and RagD mediate amino acid signaling to activate mTORC1 (1, 2). SH3BP4 (SH3 domain-binding protein 4) binds to the inactive Rag GTPase complex during amino acid starvation and prevents the association of Rag GTPase complex with mTORC1 resulting in the suppression of mTORC1 activation and cell growth (5).

  1. Sancak, Y. et al. (2008) Science 320, 1496-501.
  2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
  3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
  4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
  5. Kim, Y.M. et al. (2012) Mol Cell 46, 833-46.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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