Cell Signaling Technology

Product Pathways - Autophagy Signaling

NBR1 (D2E6) Rabbit mAb (PE Conjugate) #54516

No. Size Price
54516S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
54516 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

NBR1 (D2E6) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total NBR1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys601 of human NBR1 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated NBR1 (D2E6) Rabbit mAb #9891.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using NBR1 (D2E6) Rabbit mAb (PE Conjugate) (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (PE Conjugate) #5742 (red).

Background

Next to BRCA1 gene 1 (NBR1) protein is known for its encoding gene proximity to the BRCA1 tumor suppressor gene (1,2). N-terminal Phox and Bem1p (PB1) domains of NBR1 mediate its interaction with muscle specific titin kinase (3,4) and scaffolding protein p62 (4). NBR1 plays a role in autophagy by facilitating the autophagosomal degradation of ubiquitinated proteins independently and also in concert with p62 (5,6).

  1. Campbell, I.G. et al. (1994) Hum Mol Genet 3, 589-94.
  2. Miki, Y. et al. (1994) Science 266, 66-71.
  3. Lange, S. et al. (2005) Science 308, 1599-603.
  4. Müller, S. et al. (2006) FEBS Lett 580, 341-4.
  5. Kirkin, V. et al. (2009) Mol Cell 33, 505-16.
  6. Lamark, T. et al. (2009) Cell Cycle 8, 1986-90.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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