Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

C17orf59 (D2B5X) Rabbit mAb #54037

BLOC-1 related complex subunit 6   BORCS6   C17orf59   FLJ20014   LOC54785   PRO2472  

No. Size Price
54037S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
54037 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 42 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

C17orf59 (D2B5X) Rabbit mAb recognizes endogenous levels of total C17orf59 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu270 of human C17orf59 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using C17orf59 (D2B5X) Rabbit mAb.

Background

mTORC1 kinase complex is a critical component to regulate cell growth in response to energy levels, growth factors, and amino acids (1-4). The GTPases RagA, RagB, RagC and RagD have been shown to interact with raptor in mTORC1 (3,4). A Ragulator complex, consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, LAMTOR3/MAPKSP1, LAMTOR4/C7orf59 and LAMTOR5/HBXIP, associates with the Rag GTPases and recruit them to the surface of lysosomes (5, 6). These interactions lead to mTORC1 activation in response to amino acid signals (3-6). In addition, C17orf59 was shown to interact with Ragulator and disrupt the interaction between Ragulator and Rag GTPases (7). This disruption prevents mTOR from localizing to the lysosomal membrane and therefore inhibits mTORC1 activation (7).

  1. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
  2. Wullschleger, S. et al. (2006) Cell 124, 471-84.
  3. Sancak, Y. et al. (2008) Science 320, 1496-501.
  4. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
  5. Sancak, Y. et al. (2010) Cell 141, 290-303.
  6. Bar-Peled, L. et al. (2012) Cell 150, 1196-208.
  7. Schweitzer, L.D. et al. (2015) Cell Rep 12, 1445-55.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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