Cell Signaling Technology

Product Pathways - MAPK Signaling

SHOC2 (D7N1A) Rabbit mAb #53600

SHOC-2   SHOC2   Sur-8   Sur8  

No. Size Price
53600S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
53600 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 64 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Chicken, Bovine, Dog,

Specificity / Sensitivity

SHOC2 (D7N1A) Rabbit mAb recognizes endogenous levels of total SHOC2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SHOC2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SHOC2 (D7N1A) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human SHOC2 protein (hSHOC2-Myc/DDK; +), using SHOC2 (D7N1A) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with siRNA targeting human SHOC2 (+), using SHOC2 (D7N1A) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

SHOC2 is a scaffolding protein that harbors multiple leucine-rich repeats in tandem and is an upstream positive regulator of growth factor-dependent MAPK/ERK signaling. Research studies have demonstrated that SHOC2 forms a complex with the catalytic subunit of the PP1 phosphatase and M-Ras, and this complex drives activation of Raf-ERK signaling in response to mitogenic growth factors (1). SHOC2 has also been shown to cross-talk with and activate the PI3K/Akt signaling axis through its interaction with the p110α catalytic subunit of PI3K (2). As a positive regulator of ERK and PI3K/Akt signaling cascades, SHOC2 has been implicated in the regulation of several oncogenic cellular processes such as cell motility, invasion, and metastasis (2). A mutation in SHOC2 that introduces an N-terminal myristoylation site, promotes aberrant membrane targeting of SHOC2, hyperactive MAPK/ERK signaling, and Noonan-like syndrome (3).

  1. Rodriguez-Viciana, P. et al. (2006) Mol Cell 22, 217-30.
  2. Kaduwal, S. et al. (2015) Oncotarget 6, 33091-105.
  3. Cordeddu, V. et al. (2009) Nat Genet 41, 1022-6.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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