Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

WDR59 (D4Z7A) Rabbit mAb #53385

FLJ12270   FP977   KIAA1923   MGC11230   WD repeat domain 59   WD repeat-containing protein 59   WDR59  

No. Size Price
53385S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
53385 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 110 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

WDR59 (D4Z7A) rabbit mAb recognizes endogenous levels of total WDR59 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His356 of human WDR59 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using WDR59 (D4Z7A) Rabbit mAb.

IP

IP

Immunoprecipitation of WDR59 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is WDR59 (D4Z7A) Rabbit mAb. Western blot analysis was performed using WDR59 (D4Z7A) Rabbit mAb.

Background

The mTORC1 kinase complex plays a critical role in cell growth regulation (1, 2). mTORC1 activity is modulated by cellular environmental factors (e.g., energy levels, growth factors, amino acids) (3, 4), which are mediated through a protein complex known as GATOR (GAP Activity Towards Rags) (5). GATOR is comprised of two protein subcomplexes (GATOR1 and GATOR2), that function in opposing fashion to regulate mTORC1 activity (5). WDR59 was identified as a component of the GATOR2 subcomplex (also containing Mios, WDR24, Seh1L and Sec13), that functions to positively regulate mTORC1 activity (5). Conversely, the GATOR1 subcomplex (containing DEPDC5, Nprl2 and Nprl3), negatively regulates mTORC1 activity, through activation of RagA and RagB GTPases (5).

  1. Sancak, Y. et al. (2008) Science 320, 1496-501.
  2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
  3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
  4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
  5. Bar-Peled, L. et al. (2013) Science 340, 1100-6.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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