Product Pathways - Growth Factors/Cytokines
Mouse Epidermal Growth Factor (mEGF) #5331
|5331LC||250 µg ( With Carrier )||￥6,980.00 现货查询||购买询价|
|5331LF||250 µg ( Carrier Free )||￥6,980.00 现货查询||购买询价|
|5331SC||50 µg ( With Carrier )||￥2,386.00 现货查询||购买询价|
|5331SF||50 µg ( Carrier Free )||￥2,386.00 现货查询||购买询价|
|5331||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Source / Purification
Recombinant mouse EGF (mEGF) Asn977-Arg1029 (Accession #NP_034243) was produced in E. coli at Cell Signaling Technology.
在Cell Signaling Technology公司，在大肠杆菌中表达重组鼠源蛋白EGF (mEGF) Asn977-Arg1029 (Accession #NP_034243) 。
Recombinant mEGF has a Met on the amino terminus and has a calculated MW of 6045. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal MNSYP of recombinant mEGF was verified by amino acid sequencing.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mEGF. All lots are greater than 98% pure.
The bioactivity of recombinant mEGF was determined in an NIH/3T3 cell proliferation assay. The ED50 of each lot is between 40-250 pg/ml.
The purity of recombinant mEGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mEGF and staining overnight with Coomassie Blue.重组mEGF纯度通过SDS-PAGE验证，6µg还原（+）和未还原（-）的重组mEGF电泳后，用考马斯蓝染色过夜。
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with mEGF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).mEGF未处理或处理10分钟后的NIH/3T3 细胞提取物进行Western blot，用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® 兔单抗#4370 (上) or p44/42 MAPK (Erk1/2) (137F5) 兔单抗#4695 (下)检测.
The proliferation of NIH/3T3 cells treated with increasing concentrations of mEGF was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hr. BrdU incorporation was determined using the BrdU Cell Proliferation Assay Kit #6813 and the OD450 was determined.使用逐渐增长浓度的mEGF处理NIH/3T3细胞研究其对细胞扩增的影响，细胞使用BrdU处理4小时。BrdU掺入使用BrdU Cell Proliferation Assay Kit #6813 和OD450验证。
Less than 0.01 ng endotoxin/1 μg mEGF.
With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg mEGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.
带载体：每1 μ mEGF溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的溶液经0.22 μm滤器过滤的溶液后冻干。无载体：经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干物。
EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the ErbB/HER EGF receptor family. EGF binds to ErbB1/HER1 and induces homodimerization or induces heterodimerization with other ErbB/HER members (1). Binding of EGF signals through the MAPK, PI3K/Akt, and Stat5 pathways (1). EGF, EGF family members, EGF receptors, and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1, 2).
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- 4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb
- 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
- 6813 BrdU Cell Proliferation Assay Kit
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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