Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Epidermal Growth Factor (mEGF) #5331

No. Size Price
5331LC 250 µg ( With Carrier ) ¥6,980.00 现货查询 购买询价 防伪查询
5331LF 250 µg ( Carrier Free ) ¥6,980.00 现货查询 购买询价 防伪查询
5331SC 50 µg ( With Carrier ) ¥2,386.00 现货查询 购买询价 防伪查询
5331SF 50 µg ( Carrier Free ) ¥2,386.00 现货查询 购买询价 防伪查询
5331 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse EGF (mEGF) Asn977-Arg1029 (Accession #NP_034243) was produced in E. coli at Cell Signaling Technology.

在Cell Signaling Technology公司,在大肠杆菌中表达重组鼠源蛋白EGF (mEGF) Asn977-Arg1029 (Accession #NP_034243) 。

Molecular Characterization

Recombinant mEGF has a Met on the amino terminus and has a calculated MW of 6045. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal MNSYP of recombinant mEGF was verified by amino acid sequencing.

重组的mFGF氨基末端含有甲硫氨酸,计算得到的分子量为6045.DTT-还原和非还原状态下蛋白迁移状态与6KDa多肽相同。重组mFGF氨基端序列MNSYP通过氨基酸测序确认。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mEGF. All lots are greater than 98% pure.

6 μg还原和未还原的mEGF由SDS-PAGE测定纯度大于98%。所有批次的产品纯度均大于98%

Bioactivity

The bioactivity of recombinant mEGF was determined in an NIH/3T3 cell proliferation assay. The ED50 of each lot is between 40-250 pg/ml.

重组mEGF使用NIH/3T3细胞扩增实验检验其生物活性。每个批次的ED50值都在40-250ng/ml。

Coomassie Gel

Coomassie Gel

The purity of recombinant mEGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mEGF and staining overnight with Coomassie Blue.重组mEGF纯度通过SDS-PAGE验证,6µg还原(+)和未还原(-)的重组mEGF电泳后,用考马斯蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with mEGF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).mEGF未处理或处理10分钟后的NIH/3T3 细胞提取物进行Western blot,用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® 兔单抗#4370 (上) or p44/42 MAPK (Erk1/2) (137F5) 兔单抗#4695 (下)检测.

Bioactivity

Bioactivity

The proliferation of NIH/3T3 cells treated with increasing concentrations of mEGF was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hr. BrdU incorporation was determined using the BrdU Cell Proliferation Assay Kit #6813 and the OD450 was determined.使用逐渐增长浓度的mEGF处理NIH/3T3细胞研究其对细胞扩增的影响,细胞使用BrdU处理4小时。BrdU掺入使用BrdU Cell Proliferation Assay Kit #6813 和OD450验证。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mEGF.

1ug mEGF所含内毒素少于0.01ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg mEGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

带载体:每1 μ mEGF溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的溶液经0.22 μm滤器过滤的溶液后冻干。无载体:经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干物。

Background

EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the ErbB/HER EGF receptor family. EGF binds to ErbB1/HER1 and induces homodimerization or induces heterodimerization with other ErbB/HER members (1).  Binding of EGF signals through the MAPK, PI3K/Akt, and Stat5 pathways (1). EGF, EGF family members, EGF receptors, and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1, 2).

EGF存在于上皮细胞,成纤维细胞和许多其他的细胞类型中(1,2)。低分子量可溶型EGF由一个分子量超过130,000的跨膜前体蛋白经蛋白水解生成(1,2)。可溶型和跨膜EGF都是有活性的(2)。EGF可以诱导多种细胞包括肿瘤来源的细胞增殖,分化和存活(1-3)。EGF家族和ErbB/HER EGF受体家族有许多成员。EGF结合ErbB1/HER1诱导同源二聚化或与其他ErbB/HER成员的异源二聚化(1)。EGF,EGF家族成员,EGF受体和它们的信号通路涉及到多种癌症,可以作为介入治疗的靶点(1,2)。

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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