Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-1α (mIL-1α) #5273

il-1   IL1α   interleukin-1   interleukin1  

No. Size Price
5273LC 50 µg ( With Carrier ) ¥10,050.00 现货查询 购买询价
5273LF 50 µg ( Carrier Free ) ¥10,050.00 现货查询 购买询价
5273SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5273SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5273 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-1α (mIL-1α) Ser115-Ser270 (Accession #NP_034684) was produced in E.coli at Cell Signaling Technology.

CST公司在大肠杆菌中生产重组鼠源蛋白IL-1α (mIL-1α) Ser115-Ser270 (Accession #NP_034684)。

Molecular Characterization

Recombinant mIL-1α does not have a Met on the amino terminus and has a calculated MW of 17,990. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminus SAPYT of recombinant mIL-1α was verified by amino acid sequencing.

重组的mIL-1α 蛋白在N-末端没有Met,其分子量据推算为17,990Da。DTT-还原和未还原的蛋白迁移时是作为一个18kDa 的多肽而转移。重组mIL-1α蛋白的N-末端SAPYT的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-1α. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mIL-1α通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mIL-1α was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 3-8 pg/ml.

重组蛋白mIL-1α的生物活性是通过诱导3T3 MEFs WT细胞产生IL-6的能力实验确定的。每个批次的ED50在3-8 pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-1α was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-1α and staining overnight with Coomassie Blue.重组蛋白mIL-1α 的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The production of mouse IL-6 by 3T3 MEFs WT cultured with increasing concentrations of mIL-1α was assessed. Media from cells incubated with mIL-1α for 24 hours was collected and assayed for mouse IL-6 by ELISA and the OD450-OD650 was determined.用递增浓度的mIL-1α孵育诱导3T3 MEFs WT细胞产生IL-6实验。用mIL-1α培养细胞24小时并收集上清,鼠源IL-6用ELISA 实验测定,并测定OD450-OD650 数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from 3T3 MEFs WT untreated or treated with mIL-1α for 10 minutes, using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (upper) and p38 MAPK Antibody #9212 (lower).Western免疫印迹。用Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215(上图)和 p38 MAPK Antibody #9212 (下图)研究未经处理和经mIL-1α 处理10分钟的3T3 MEFs WT 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-1α.

内毒素含量:<0.01 ng /1 μg mIL-1α。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-1α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mIL-1α蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-1α蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-1α is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes and epithelial cells (1). IL-1α is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL-1α are primarily retained intracellularly rather than constitutively secreted. (1,2). Signaling by IL-1α involves IL-1α binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NFκB pathways (1,2). IL-1α also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. Inhibition of IL-1α activity is through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal. IL-1α has been shown to be a key mediator of virus-induced inflammatory responses in mice (3).

IL-1α是促炎症因子,主要由激活的单核细胞、淋巴细胞核上皮细胞产生(1)。IL-1α作为有活性的前体而合成,但是经过细胞质的蛋白酶剪切形成成熟形式(1,2)。通常来讲,前体和成熟形式的IL-1α主要是保留在细胞内而不是组成型的分泌(1,2)。通过IL-1α的信号由IL-1α结合到IL-1的附属蛋白(IL-1-AcP),然后此复合体结合到IL-1RI (1,2)。信号通路的发生要通过激活MAP 激酶和NF-κB信号通路(1,2)。IL-1α也能结合到缺少胞内信号域的IL-RII上,并因此作为高亲和性的诱捕受体。IL-1α活性被抑制是通过IL-1R拮抗剂(IL-1Ra),它结合IL-1R1但是不发生信号。在小鼠中。IL-1α是主要的病毒诱导的炎症应答的转化者(3)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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