Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-10 (mIL-10) #5261

No. Size Price
5261LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
5261LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
5261SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5261SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5261 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-10 (mIL-10) Ser19 - Ser178 (Accession # NP_034678) was produced in E. coli at Cell Signaling Technology.

CST公司生产的鼠源重组蛋白IL-10 (mIL-10) Ser19 - Ser178 (Accession # NP_034678)是从大肠杆菌细胞表达而来。

Molecular Characterization

Recombinant mIL-10 does not have a Met on the amino terminus and has a calculated MW of 18,976. DTT-reduced and non-reduced protein migrate as 19 kDa polypeptides. The expected amino-terminal SRGQY of recombinant mIL-10 was verified by amino acid sequencing.

重组的mIL-10 蛋白在N-末端没有Met标签,蛋白分子量据推算为18,976Da。DTT-还原蛋白和未还原蛋白作为19kDa的多肽转移。重组mIL-10 蛋白的N-末端SRGQY的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-10. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) 重组mIL-10通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mIL-10 was determined in a MC/9 cell proliferation assay. The ED50 of each lot is between 0.3-1.3 ng/ml.

mIL-10 的生物活性是通过MC/9细胞增殖实验来确定的。每个批次的ED50在 0.3-1.3 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-10 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-10 and staining overnight with Coomassie Blue.重组mIL-10蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-10蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of MC/9 cells treated with increasing concentrations of mIL-10 in the presence of 1 pg/ml mouse IL-4 (#5208) was assessed. After 72 hour treatment with mIL-10 cells, were incubated with a tetrazolium salt and the OD450-OD650 was determined.在10 pg/ml鼠源IL-4(#5208) 存在下mIL-10蛋白浓度递增条件下研究MC/9细胞增殖实验。用mIL-10培养细胞72小时后用四唑盐孵育。并测定OD450 - OD650值。

Western Blotting

Western Blotting

Western blot analysis of extracts from MC/9 cells, untreated or treated with mIL-10 for 20 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).Western免疫印迹。用Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb #9145(上图)和Stat1 Stat3 Antibody #9132 (下图) 研究未经处理的和经mIL-10 处理20 min的MC/9 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-10.

每微克mIL-10内毒素含量小于0.01 ng。

Formulation

With carrier: A 0.22 μm filtered solution of 0.33 mg/ml mIL-10 in PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-10. Carrier free: A 0.22 μm filtered solution of 0.33 mg/ml mIL-10 in PBS, pH 7.2.

有载体:每微克mIL-10蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-10蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-10 is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1). Binding of IL-10 leads to the activation of Jak1 and Tyk2, which phosphorylates Stat3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFNγ receptor, by upregulating expression of SOCS1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (6).

IL-10 是抗炎症的细胞因子,它由T细胞,NK细胞和巨噬细胞产生(1,2)。IL-10通过结合细胞表面受体复合体包括 IL-10 RI和 IL-10 RII而起始信号转导(1)。结合IL-10导致Jak1和Tyk2的激活, 继而磷酸化Stat-3 (1,3)。IL-10的抗炎症活性是由于它能通过其他的细胞因子受体而阻止细胞传递,特别是通过上调SOCS-1的表达而抑制IFNγ受体(1,3)。除此之外, IL-10通过抑制酪氨酸磷酸化CD28而促进T细胞耐受性(4,5)。IL-10是免疫应答的重要负调控因子,这能够维持怀孕状态(1)。相反,增加IL-10水平对利什曼虫的持续感染有很大的帮助(6)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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