Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-13 (mIL-13) #5242

IL13   T-cell activation protein P600  

No. Size Price
5242SC 5 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
5242SF 5 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
5242 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-13 (mIL-13) Ser26-Phe131 (Accession #NP_032381) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的小鼠重组蛋白IL-13 (mIL-13) Ser26-Phe131 (Accession #NP_032381)是从人的293细胞表达而来。

Molecular Characterization

Recombinant mIL-13 contains no "tags" and the nonglycosylated protein has a calculated MW of 11,677. DTT-reduced and non-reduced protein migrate as 12-20 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal SVSLP of recombinant mIL-13 was verified by amino acid sequencing.

重组的mIL-13 蛋白没有标签,未糖基化的蛋白分子量据推算为11,677Da。DTT-还原和未还原的蛋白分子作为12-20 kDa的蛋白而迁移。在SDS-PAGE中具有稍低的迁移率是由于糖基化。重组mIL-13蛋白的N-末端SVSLP的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-13. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) 重组mIL-13通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mIL-13 was determined in a B9 cell proliferation assay. The ED50 of each lot is between 0.8 - 4 ng/ml.

mIL-13 的生物活性是通过B9细胞增殖实验来确定的。每个批次的ED50在0.8 - 4 ng/ml之间。

Bioactivity

Bioactivity

The proliferation of B9 cells treated with increasing concentrations of mIL-13 was assessed. After 48 hour treatment with mIL-13, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在mIL-13蛋白浓度递增条件下研究B9细胞增殖实验来确定其生物活性。用mIL-13培养细胞48小时后用四唑盐孵育。并测定OD450 - OD650值。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-13 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-13 and staining overnight with Coomassie Blue.重组mIL-13 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-13蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from TF-1 cells untreated or treated with mIL-13 for 10 minutes, using Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb Antibody #9364 (upper) and Stat6 Antibody #9362 (lower).Western免疫印迹。用Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb Antibody #9364 (上图)和Stat6 Antibody #9362 (下图) 研究未经处理的和经mIL-13 处理10 min的TF-1 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1μg mIL-13.

每微克mIL-13内毒素含量小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-13. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mIL-13蛋白溶解在包含 20 μg BSA的PBS(pH 7.2) 溶液中,并通过 0.22 μm 滤膜冻干。

无载体: 每微克mIL-13蛋白溶解在PBS(pH 7.2) 溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-13 is produced by T cells and is important in the TH2 response. IL-13 targets include B cells, eosinophils, fibroblasts, mast cells and macrophages (1-3). IL-13 binds specifically to IL-13Rα1 that complexes with IL-4Rα to form the Type II IL-4R. Jak1 and Tyk2 are activated and signal through Stat3 and Stat6 (4). IL-13Rα2 is a different gene product, lacks the intracellular domain, does not complex with IL-4Rα and does not signal (1,4,5). The extracellular domain of IL-13Rα2 is often elevated in diseased states. IL-13 plays key roles in airway hyperresponsiveness (AHR) of allergic asthma (1,6,7) and modulates resistance to parasitic organisms (1).

IL-13由T细胞产生并且在TH2应答中非常重要。IL-13的目标包括B细胞、嗜酸性粒细胞、成纤维细胞、肥大细胞和巨噬细胞(1-3)。IL-13特异性的结合到IL-13Rα1并与IL-4Rα 形成II类 IL-4R复合体。Jak1和 Tyk2 的激活和信号转导是通过Stat3和Stat6 (6)。 IL-13Rα2是不同的基因产物,缺乏细胞内结构域,不与IL-4Rα形成复合体并且也不进行信号转导(1,6,7)。 IL-13Rα2的胞外域通常在疾病状态下升高表达。IL-13在气道高反应性(AHR)过敏性哮喘(1,4,5)和改变对寄生生物的抵抗中发挥重要作用(1)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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