Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Neurotrophin-3 (hNT-3) #5237

No. Size Price
5237SC 10 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
5237SF 10 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
5237 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human Neurotrophin-3 (hNT-3) Tyr139-Thr257 (Accession #NP_002518) was produced in E.coli at Cell Signaling Technology.

重组人神经营养因子3(hNT-3)Tyr139-Thr257(Accession #NP_002518)由CST公司利用大肠杆菌生产。

Molecular Characterization

Recombinant hNT-3 does not have a Met on the amino terminus and has a calculated MW of 13,625. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal YAEHK of recombinant hNT-3 was verified by amino acid sequencing.

重组hNT-3在氨基末端没有Met,分子量为13,625。DTT还原和非还原的蛋白电泳可见14kDa的多肽。重组hNT-3的氨基末端YAEHK使用氨基酸测序验证。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hNT-3. All lots are greater than 98% pure.

6μg还原和非还原的重组hNT-3经SDS-PAGE验证纯度>98%。 每个批次的纯度超过98%。

Bioactivity

The bioactivity of recombinant hNT-3 was determined in a TrkC-transfected NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.5 - 5 ng/ml.

重组hNT-3的生物活性使用TrkC转染的NIH/3T3细胞增殖分析进行确定。每个批次的ED50为0.5-5ng/ml。

Coomassie Gel

Coomassie Gel

The purity of recombinant hNT-3 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hNT-3 and staining overnight with Coomassie Blue.

重组hNT-3的纯度由6ug 还原(+)和非还原(-)的重组hNT-3 SDS-PAGE和与考马斯蓝染色过夜确定。

Bioactivity

Bioactivity

The ability of hNT-3 to induce phosphorylation of TrkB was assessed. After starvation, TrkB-transfected NIH/3T3 cells were treated with increasing concentrations of hNT-3 for 5 minutes. Cells were lysed, and phospho-TrkB was quatified using PathScan® Phospho-TrkB (panTyr) Sandwhich ELISA Kit #7108. OD450-OD650 is shown.

hNT-3 诱导磷酸化TrkB的能力已评估。饥饿处理后,转染TrkB的NIH/3T3细胞用更高浓度的hNT-3处理5分钟。细胞裂解,phospho-TrkB用PathScan®Phospho-TrkB (panTyr) Sandwhich ELISA Kit #7108进行测定。OD450-OD650可见。

Bioactivity

Bioactivity

The proliferation of TrkC-transfected NIH/3T3 cells treated with increasing concentrations of hNT-3 was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.

用增加浓度的hNT-3处理TrkC转染的NIH/3T3细胞,该细胞的增殖能力已评估。处理24小时后,细胞用BrdU标记4小时。BrdU的嵌入由ELISA OD450-OD690确定。

Western Blotting

Western Blotting

Western blot analysis of extracts from TrkB-transfected NIH/3T3 cells untreated and treated with hNT-3 for 5 minutes, using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 (upper) or TrkB (80E3) Rabbit mAb #4603 (lower).

Western blot分析转染了TrkB的NIH/3T3细胞,未处理和hNT-3处理5分钟,使用的抗体是Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb 兔单抗#4621(上图)和TrkB (80E3) Rabbit mAb 兔单抗#4603(下图)。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hNT-3.

内毒素小于0.01ng/1 μg hNT-3。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM phosphate, pH 8.0 containing 500 mM NaCl and 20 μg BSA per 1 μg hNT-3. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM phosphate, pH 8.0 containing 500 mM NaCl.

有载体:冻干自0.22μm孔过滤的20 mM 磷酸盐,pH为8.0,每μg hNT-3含有500mM NaCl和20μg BSA。无载体:冻干自0.22μm孔过滤的20 mM 磷酸盐,pH 为8.0,含500 mM NaCl。

Background

NT-3 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-4 (1). NT-3 is expressed in a number of cell types including neuronal cells, eosinophils, and melanocytes (1-3). NT-3 is required for the development of peripheral sensory neurons (4). NT-3 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1). NT-3 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkC. While all neurotrophins bind to the p75NTR receptor, NT-3 preferentially binds to TrkC and, with lesser affinity, TrkB (1).

NT-3是一种与神经营养因子家族结构相关的蛋白质,其中包括β-神经生长因子,BDNF和NT-4(1)。NT-3在某些类型的细胞中表达,包括神经细胞,嗜酸性粒细胞,黑色素细胞(1-3)。NT-3是外周感觉神经元发育所需要的(4)。NT-3被细胞以前体蛋白的形式分泌,被裂解成成熟的形式(1)。NT-3信号是通过两种不同的受体介导,它们是神经营养因子受体p75NTR和Trk酪氨酸激酶受体TrkC。虽然所有的神经营养因子都能结合p75NTR受体,NT-3能优先结合TrkC,也能以较低的亲和力结合TrkB受体(1)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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