Product Pathways - Growth Factors/Cytokines
Human Neurotrophin-3 (hNT-3) #5237
|5237SC||10 µg ( With Carrier )||￥2,580.00 现货查询||购买询价|
|5237SF||10 µg ( Carrier Free )||￥2,580.00 现货查询||购买询价|
|5237||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Source / Purification
Recombinant human Neurotrophin-3 (hNT-3) Tyr139-Thr257 (Accession #NP_002518) was produced in E.coli at Cell Signaling Technology.
Recombinant hNT-3 does not have a Met on the amino terminus and has a calculated MW of 13,625. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal YAEHK of recombinant hNT-3 was verified by amino acid sequencing.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hNT-3. All lots are greater than 98% pure.
The bioactivity of recombinant hNT-3 was determined in a TrkC-transfected NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.5 - 5 ng/ml.
The purity of recombinant hNT-3 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hNT-3 and staining overnight with Coomassie Blue.
重组hNT-3的纯度由6ug 还原(+)和非还原(-)的重组hNT-3 SDS-PAGE和与考马斯蓝染色过夜确定。
The ability of hNT-3 to induce phosphorylation of TrkB was assessed. After starvation, TrkB-transfected NIH/3T3 cells were treated with increasing concentrations of hNT-3 for 5 minutes. Cells were lysed, and phospho-TrkB was quatified using PathScan® Phospho-TrkB (panTyr) Sandwhich ELISA Kit #7108. OD450-OD650 is shown.
hNT-3 诱导磷酸化TrkB的能力已评估。饥饿处理后，转染TrkB的NIH/3T3细胞用更高浓度的hNT-3处理5分钟。细胞裂解，phospho-TrkB用PathScan®Phospho-TrkB (panTyr) Sandwhich ELISA Kit #7108进行测定。OD450-OD650可见。
The proliferation of TrkC-transfected NIH/3T3 cells treated with increasing concentrations of hNT-3 was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.
Western blot analysis of extracts from TrkB-transfected NIH/3T3 cells untreated and treated with hNT-3 for 5 minutes, using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 (upper) or TrkB (80E3) Rabbit mAb #4603 (lower).
Western blot分析转染了TrkB的NIH/3T3细胞，未处理和hNT-3处理5分钟，使用的抗体是Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb 兔单抗#4621（上图）和TrkB (80E3) Rabbit mAb 兔单抗#4603（下图）。
Less than 0.01 ng endotoxin/1 μg hNT-3.
内毒素小于0.01ng/1 μg hNT-3。
With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM phosphate, pH 8.0 containing 500 mM NaCl and 20 μg BSA per 1 μg hNT-3. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM phosphate, pH 8.0 containing 500 mM NaCl.
有载体：冻干自0.22μm孔过滤的20 mM 磷酸盐，pH为8.0，每μg hNT-3含有500mM NaCl和20μg BSA。无载体：冻干自0.22μm孔过滤的20 mM 磷酸盐，pH 为8.0，含500 mM NaCl。
NT-3 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-4 (1). NT-3 is expressed in a number of cell types including neuronal cells, eosinophils, and melanocytes (1-3). NT-3 is required for the development of peripheral sensory neurons (4). NT-3 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1). NT-3 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkC. While all neurotrophins bind to the p75NTR receptor, NT-3 preferentially binds to TrkC and, with lesser affinity, TrkB (1).
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- 4603 TrkB (80E3) Rabbit mAb
- 4621 Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb
- 7108 PathScan® Phospho-TrkB (panTyr) Sandwich ELISA Kit
- 9872 Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile)
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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