Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Interleukin-1α (hIL-1α) #5236

hIL-1   hIL-1alpha   IL-1   IL-1a   il-1alpha   Interleukin 1alpha   interleukin1  

No. Size Price
5236SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5236SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5236 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human IL-1α (hIL-1α) Ser113-Ala271 (Accession #NP_000566) was produced in E. coli at Cell Signaling Technology.

CST公司在大肠杆菌中生产重组人蛋白IL-1α (hIL-1α) Ser113-Ala271 (Accession #NP_000566)。

Molecular Characterization

Recombinant hIL-1α does not have a Met on the amino terminus and has a calculated MW of 18,063. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal SSPFS of recombinant hIL-1α was verified by amino acid sequencing.

重组的hIL-1α 蛋白在N-末端没有Met,其分子量据推算为18,063Da。DTT-还原和未还原的蛋白迁移时是作为一个18kDa 的多肽而转移。重组hIL-1α 蛋白的N-末端SSPFS的序列通过测序得到。


>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-1α. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) hIL-1α通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。


The bioactivity of recombinant hIL-1α was determined by its ability to induce IL-8 production by primary human fibroblasts. The ED50 of each lot is between 5-15 pg/ml.

重组蛋白hIL-1α 的生物活性是通过诱导人的成纤维元代细胞产生IL-8的能力实验确定的。每个批次的ED50在5-15pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hIL-1α was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-1α and staining overnight with Coomassie Blue.重组蛋白hIL-1α 的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated or treated with hIL-1α for 15 minutes, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 (E498) Antibody #3987 (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (上图)和NF-κB p65 (E498) Antibody #3987 (下图)研究未经处理和经hIL-1α 处理10分钟的MCF7细胞的细胞提取液。



The production of human IL-8 by primary human fibroblasts cultured with increasing concentrations of hIL-1α was assessed. Media from cells incubated with hIL-1α for 24 hours was collected and assayed for human IL-8 by ELISA and the OD450-OD650 was determined.用递增浓度的hIL-1α孵育诱导人成纤维原代细胞产生IL-8实验。用hIL-1α培养细胞24小时并收集上清,人IL-8用ELISA 实验测定,并测定OD450-OD650 数值。


Less than 0.01 ng endotoxin/1 μg hIL-1α.

内毒素含量:<0.01 ng /1 μg hIL-1α。


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 4mM DTT and 20 μg BSA per 1 μg hIL-1α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 4mM DTT.

有载体: 每微克hIL-1α蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克hIL-1α蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。


IL-1α is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes, and epithelial cells (1). IL-1α is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL-1α are primarily retained intracellularly rather than constitutively secreted. (1,2). Signaling by IL-1α involves IL-1α binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling occurs through activation of MAP kinase and NF-κB pathways (1,2). IL-1α also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1α activity is inhibited through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal. IL-1α has been shown to be a key mediator of virus-induced inflammatory responses in mice (3).

IL-1α是促炎症因子,主要由激活的单核细胞、淋巴细胞核上皮细胞产生(1)。IL-1α作为有活性的前体而合成,但是经过细胞质的蛋白酶剪切形成成熟形式(1,2)。通常来讲,前体和成熟形式的IL-1α主要是保留在细胞内而不是组成型的分泌(1,2)。通过IL-1α的信号由IL-1α结合到IL-1的附属蛋白(IL-1-AcP),然后此复合体结合到IL-1RI (1,2)。信号通路的发生要通过激活MAP 激酶和NF-κB信号通路(1,2)。IL-1α也能结合到缺少胞内信号域的IL-RII上,并因此作为高亲和性的诱捕受体。IL-1α活性被抑制是通过IL-1R拮抗剂(IL-1Ra),它结合IL-1R1但是不发生信号。在小鼠中。IL-1α是主要的病毒诱导的炎症应答的转化者(3)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :