Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Interleukin-1α (hIL-1α) #5236

hIL-1   hIL-1alpha   IL-1   IL-1a   il-1alpha   Interleukin 1alpha   interleukin1  

No. Size Price
5236SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5236SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5236 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human IL-1α (hIL-1α) Ser113-Ala271 (Accession #NP_000566) was produced in E. coli at Cell Signaling Technology.

CST公司在大肠杆菌中生产重组人蛋白IL-1α (hIL-1α) Ser113-Ala271 (Accession #NP_000566)。

Molecular Characterization

Recombinant hIL-1α does not have a Met on the amino terminus and has a calculated MW of 18,063. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal SSPFS of recombinant hIL-1α was verified by amino acid sequencing.

重组的hIL-1α 蛋白在N-末端没有Met,其分子量据推算为18,063Da。DTT-还原和未还原的蛋白迁移时是作为一个18kDa 的多肽而转移。重组hIL-1α 蛋白的N-末端SSPFS的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-1α. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) hIL-1α通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant hIL-1α was determined by its ability to induce IL-8 production by primary human fibroblasts. The ED50 of each lot is between 5-15 pg/ml.

重组蛋白hIL-1α 的生物活性是通过诱导人的成纤维元代细胞产生IL-8的能力实验确定的。每个批次的ED50在5-15pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hIL-1α was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-1α and staining overnight with Coomassie Blue.重组蛋白hIL-1α 的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated or treated with hIL-1α for 15 minutes, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 (E498) Antibody #3987 (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (上图)和NF-κB p65 (E498) Antibody #3987 (下图)研究未经处理和经hIL-1α 处理10分钟的MCF7细胞的细胞提取液。

Bioactivity

Bioactivity

The production of human IL-8 by primary human fibroblasts cultured with increasing concentrations of hIL-1α was assessed. Media from cells incubated with hIL-1α for 24 hours was collected and assayed for human IL-8 by ELISA and the OD450-OD650 was determined.用递增浓度的hIL-1α孵育诱导人成纤维原代细胞产生IL-8实验。用hIL-1α培养细胞24小时并收集上清,人IL-8用ELISA 实验测定,并测定OD450-OD650 数值。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-1α.

内毒素含量:<0.01 ng /1 μg hIL-1α。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 4mM DTT and 20 μg BSA per 1 μg hIL-1α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 4mM DTT.

有载体: 每微克hIL-1α蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克hIL-1α蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-1α is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes, and epithelial cells (1). IL-1α is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL-1α are primarily retained intracellularly rather than constitutively secreted. (1,2). Signaling by IL-1α involves IL-1α binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling occurs through activation of MAP kinase and NF-κB pathways (1,2). IL-1α also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1α activity is inhibited through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal. IL-1α has been shown to be a key mediator of virus-induced inflammatory responses in mice (3).

IL-1α是促炎症因子,主要由激活的单核细胞、淋巴细胞核上皮细胞产生(1)。IL-1α作为有活性的前体而合成,但是经过细胞质的蛋白酶剪切形成成熟形式(1,2)。通常来讲,前体和成熟形式的IL-1α主要是保留在细胞内而不是组成型的分泌(1,2)。通过IL-1α的信号由IL-1α结合到IL-1的附属蛋白(IL-1-AcP),然后此复合体结合到IL-1RI (1,2)。信号通路的发生要通过激活MAP 激酶和NF-κB信号通路(1,2)。IL-1α也能结合到缺少胞内信号域的IL-RII上,并因此作为高亲和性的诱捕受体。IL-1α活性被抑制是通过IL-1R拮抗剂(IL-1Ra),它结合IL-1R1但是不发生信号。在小鼠中。IL-1α是主要的病毒诱导的炎症应答的转化者(3)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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