Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Betacellulin (hBTC) #5235

No. Size Price
5235LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
5235LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
5235SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5235SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5235 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human Betacellulin (hBTC) Asp31-Tyr111 (Accession #P35070) was produced in E. coli at Cell Signaling Technology.

CST在大肠杆菌中生产重组人蛋白Betacellulin (hBTC) Asp31-Tyr111 (Accession #P35070)。

Molecular Characterization

Recombinant hBTC does not have a Met at the amino terminus and has a calculated MW of 8,984. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal DGNST of recombinant hBTC was verified by amino acid sequencing.

重组的hBTC蛋白在N-末端没有Met标签,其分子量据推算为8,984Da。DTT-还原和未还原的蛋白迁移时是作为一个14kDa的多肽而转移。重组hBTC蛋白的N-末端DGNST的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hBTC. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) hBTC通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant hBTC was determined in a MCF 10A cell proliferation assay. The ED50 of each lot is between 0.6 and 1.0 ng/ml.

重组蛋白hBTC的生物活性是同过MCF 10A细胞的细胞增殖实验确定的。每个批次的ED50在0.6-1ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hBTC was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hBTC and staining overnight with Coomassie Blue.重组蛋白hBTC 的纯度用6 µg 还原 (+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of MCF 10A cells treated with increasing concentrations of hBTC was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hr. BrdU incorporation was determined by ELISA and the OD450 was determined.经过递增浓度的hBTC处理后MCF 10A 细胞的增殖能力。hBTC处理MCF 10A 细胞24小时,用BrdU标记4小时,BrdU的结合通过ELISA和OD450值来确定。

Bioactivity

Bioactivity

The ability of hBTC to induce phosphorylation of p44/42 MAPK (Erk1/2) was assessed. After starvation, MCF 10A cells were treated with increasing concentrations of hBTC for 10 min. Cells were lysed, and Phospho-p44/42 MAPK (Erk1/2) was quantified using PathScan® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwhich ELISA Kit #7177. OD450 is shown.hBTC 诱导磷酸化的p44/42 MAPK (Erk1/2)能力的实验。 记过饥饿处理后, MCF 10A 细胞用递增浓度的hBTC处理10分钟。然后裂解。磷酸化的p44/42 MAPK (Erk1/2) 用PathScan® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwhich ELISA Kit #7177来确定。 OD450 数值也有显示。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hBTC.

内毒素含量:<0.01 ng/1 μg hBTC。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hBTC. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克hBTC蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克hBTC蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

Betacellulin (BTC), a member of the EGF family of ligands, is a widely expressed growth factor that was originally identified in conditioned medium of a mouse pancreatic beta cell line (1). BTC is synthesized as a 178 amino acid transmembrane precursor protein that is proteolytically cleaved into an 80 amino acid mature protein (1). Human BTC shares 74% amino acid sequence identity with mouse BTC (1). BTC can bind to and activate the ErbB family of receptor tyrosine kinases, EGFR/ErbB1, HER2/ErbB2, HER3/ErbB3, and HER4/ErbB4 (1,2). BTC can also bind to and activate EGFR/ErbB1 and HER4/ErbB4 homodimers (1). Ligand-receptor interactions result in the activation of the PI3K and Erk pathways (1). BTC induces the proliferation of pancreatic beta cell lines in vitro (2). Moreover, research studies have shown that BTC induces beta cell regeneration and increased insulin secretion in rodent models of diabetes (2-4).

乙胞素 (BTC),EGF家族成员的配体,是广泛表达的生长因子,它最初是从小鼠胰腺 beta细胞系的条件型培养基中发现鉴定(1)。BTC合成一个178个氨基酸的跨膜前体蛋白并被蛋白酶水解剪切为80个氨基酸的成熟蛋白(1)。人的BTC与小鼠BTC具有74%的相似度(1)。BTC能结合到并激活ErbB家族的受体酪氨酸激酶EGFR/ErbB1, HER2/ErbB2, HER3/ErbB3 和 HER4/ErbB4 (1,2)。BTC也能够结合到并激活EGFR/ErbB1和HER4/ErbB4同型二聚体(1)。配体-受体的相互作用导致了PI3K和Erk通路的激活(1)。在体外,BTC诱导了胰腺beta细胞系的增殖(2)。此外, 研究结果也表明了在啮齿动物的肥胖模型中BTC诱导beta细胞再生并增加了胰岛素的分泌(2-4)。

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

PathScan is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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