Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human FGF acidic (hFGF acidic) #5234

No. Size Price
5234LC 50 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5234LF 50 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5234SC 10 µg ( With Carrier ) ¥1,302.00 现货查询 购买询价
5234SF 10 µg ( Carrier Free ) ¥1,302.00 现货查询 购买询价
5234 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human FGF acidic (hFGF acidic) Phe16-Asp155 (Accession #NP_000791) was produced in E.coli at Cell Signaling Technology.

Cell Signaling Technology使用大肠杆菌表达人重组酸性FGF蛋白(hFGF acidic) Phe16-Asp155 (Accession #NP_000791)。

Molecular Characterization

Recombinant hFGF acidic does not have a Met on the amino terminus and has a calculated MW of 15,835. DTT-reduced and non-reduced protein migrate as 16 kDa polypeptides. The expected amino-terminal FNLPP of recombinant hFGF acidic was verified by amino acid sequencing.

重组的hFGF羧基端不含甲硫氨酸,分子量为12,835.DTT-还原和非还原状态下蛋白迁移速率与16 kDa的多肽相当。预测的重组hFGF氨基端序列通过氨基酸测序确认。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hFGF acidic. All lots are greater than 98% pure.

6 μg还原和未还原的mEGF由SDS-PAGE测定纯度大于98%。所有批次的产品纯度均大于98%。

Bioactivity

The bioactivity of recombinant hFGF acidic was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 7-20 pg/ml.

重组hFGF的生物活性通过NIH/3T3细胞增殖实验而确认。每一批次的ED50均在7-20 pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hFGF acidic was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hFGF acidic and staining overnight with Coomassie Blue.重组hFGF acidic的纯度使用SDS-PAGE进行检测,6 µg 还原(+)和未还原(-)重组hFGF acidic电泳后使用考马斯蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of NIH/3T3 cells treated with increasing concentrations of hFGF acidic was assessed in the presence of 10 µg/ml heparin. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.在10 µg/ml 肝素的存在下,使用逐渐增长浓度的hFGF acidic处理NIH/3T3检测细胞扩增效率。处理24小时候,细胞使用BrdU标记4小时。BrdU掺入使用ELISA和OD450-OD690 检测。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with human FGF acidic for 10 minutes, using Phospho-CREB (Ser133) Antibody #9191 (upper) or CREB Antibody #9192 (lower).人FGF acidic处理NIH/3T3 10分钟或不经处理,获得细胞提取物进行Western blot分析,使用Phospho-CREB (Ser133) 抗体#9191(上)或CREB抗体#9192(下)进行检测。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hFGF acidic.

1ug 酸性hFGF蛋白所含内毒素低于0.01ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hFGF acidic. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

带载体:每1 μg 酸性hFGF溶于含有20 μgBSA、pH7.2的PBS溶液经0.22 μm滤器过滤后冻干。无载体:溶于pH7.2的PBS溶液经0.22 μm滤器过滤获得冻干物。

Background

FGF acidic is a potent growth factor for fibroblasts and endothelial cells (1). FGF acidic is involved in wound repair, angiogenesis, and development (1). FGF acidic is secreted from cells via an endoplasmic reticulum/Golgi independent mechanism (1,2). The ability of FGF acidic to bind to heparin sulfate is required for its ability to interact with FGF receptors and induce signaling (1-4). There are four distinct FGF receptors and each has multiple splice variants (1,3). FGF acidic binds with high affinity to many, but not all, FGFRs (1). Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways (1).

酸性FGF是一种成纤维细胞和内皮细胞的潜在生长因子(1)。酸性FGF涉及损伤修复,血管生长和发育(1)。酸性FGF由细胞通过内质网/高尔基体的非依赖机制分泌产生(1,2)。酸性FGF与硫酸肝素的结合能力对于它与FGF受体结合并激活下游信号是必须的(1-4)。有4种不同的FGF受体,每一种都有多个剪切异构体(1,3)。酸性FGF与大部分但不是所有的FGFR有高亲和性(1)。酸性FGF结合FGFR激活的型号通路包括ras-raf-MAPK, PLCγ/PKC, 和PI3K/Akt信号通路(1)。

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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