Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Transforming Growth Factor β1 (mTGF-β1) #5231

LAP   Latency-associated peptide   TGF beta-1   TGF-b   TGF-beta   TGFb   TGFb1   TGFbeta   tgfbeta1  

No. Size Price
5231LC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5231LF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5231 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse TGF-β1 (hTGF-β1) Ala279-Ser390 (Accession #NP_035707) was expressed in human 293 cells at Cell Signaling Technology.

Cell Signaling Technology公司使用人293T细胞表达小鼠重组蛋白TGF-β1 (hTGF-β1) Ala279-Ser390 (编号 #NP_035707)。

Molecular Characterization

Recombinant mTGF-β1 contains no "tags" and the nonglycosylated protein has a calculated MW of 12,810. DTT-reduced protein migrates as a 13 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 25 kDa protein. The expected amino-terminal ALDTN of recombinant mTGF-β1 was verified by amino acid sequencing.重组mTGF-β1蛋白不带标记且非糖基化的蛋白分子量为12,810。DTT-还原状态下蛋白迁移状态与13KDa多肽相同,非还原状态以半胱氨酸连接形成的同二聚体与25kDa蛋白相同。重组mTGF-β1氨基端序列ALDTN通过氨基酸测序确认。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mTGF-β1. All lots are greater than 98% pure.6 μg还原和未还原的mEGF由SDS-PAGE测定纯度大于98%。所有批次的产品纯度均大于98%

Bioactivity

The bioactivity of recombinant mTGF-β1 was determined by assessing inhibition of IL-4 induced HT-2 cell proliferation. The ED50 of each lot is between 40-200 pg/ml.mTGF-β1的活性通过检测其对IL-4诱导的HT-2细胞增殖的抑制能力来确定。

Bioactivity

Bioactivity

The inhibition of IL-4 induced proliferation in HT-2 cells treated with increasing concentrations of mTGF-β1 was assessed. After 48 hour treatment with hTGF-β1, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.使用逐渐增加浓度的mTGF-β1抑制IL-4诱导的HT-2 的增殖。hTGF-β1处理48小时候,细胞使用一种四唑盐处理然后用OD450 - OD650 检测。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-2 cells, untreated or treated with the mTGF-β1 for 20 minutes, using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) and Smad2 (D43B4) XP® Rabbit mAb #5339 (lower).mTGF-β1 处理HT-2细胞20分钟或不处理,获得细胞提取物进行Western blot,用Phospho-Smad2 (Ser465/467) (138D4)兔源单克隆抗体#3108 (上) 和Smad2 (D43B4) XP® 兔源单克隆抗体 #5339 (下)检测.

Coomassie Gel

Coomassie Gel

The purity of recombinant mTGF-β1 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mTGF-β1 and staining overnight with Coomassie Blue.使用SDS-PAGE验证重组mTGF-β1 的纯度,6µg还原(+)与未还原(-)的重组mTGF-β1电泳后用考马斯蓝染色过夜。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mTGF-β1.1ug mTGF-β1所含内毒素低于0.01ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg mTGF-β1. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.带载体:每1 μg mTGF-β1溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的溶液经0.22 μm滤器过滤后冻干。无载体:经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干物。

Background

TGF-β1 activities include proliferation, angiogenesis, and promotion or inhibition of many immune events (1-3). TGF-β1 is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells (3). TGF-β1 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,4). The oligomeric receptor complex phosphorylates subsets of the Smad proteins that then act to induce and repress a number of target genes (1,3,4). TGF-β1 binding can also activate the Erk2, p38, and JNK pathways via TAK1 (4). TGF-β1 appears to promote late stage progression and metastasis in some cancers (1,2).

TGF-β1的功能包括促进增殖,血管生成,和引发或抑制许多免疫活动(1-3)。TGF-β1可以被多种细胞生成,包括调节性T细胞,成纤维细胞,上皮细胞和内皮细胞(3)。TGF-β1与TβRII同二聚体结合,随后与TβRI同二聚体形成复合物(1,4)。这种低聚物受体复合物磷酸化Smad蛋白随后诱导或抑制一系列靶基因(1,3,4)。TGF-β1也可以通过TAK1激活Erk2,p38和JNK信号通路(4)。TGF-β1可以在某些癌症中促进晚期恶化和转移(1,2)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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