Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Macrophage Colony Stimulating Factor (mM-CSF) #5228

No. Size Price
5228LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
5228LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
5228SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5228SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5228 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse M-CSF (mM-CSF) Lys33-Glu262 (Accession #NP_031804) was expressed in human 293 cells at Cell Signaling Technology.

CST公司在人293细胞中生产重组鼠蛋白M-CSF (mM-CSF) Lys33-Glu262 (Accession #NP_031804)。

Molecular Characterization

Recombinant mM-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 25,987. DTT-reduced protein migrates as a 37-50 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 55-80 kDa protein. Heterogeneity and lower mobility in SDS-PAGE are due to glycosylation. The expected amino-terminal KEVSE of recombinant mM-CSF was verified by amino acid sequencing.

重组的mM-CSF蛋白在N-末端没有标签,未糖基化蛋白分子量据推算为25,987Da。DTT-还原的蛋白作为37-50kDa多肽而转移,未还原的蛋白经二硫键连接成同源二聚体迁移时是作为一个55-80 kDa的蛋白而转移,异源二聚体在SDS-PAGE胶中稍低的迁移率是由于糖基化。重组mM-CSF蛋白的N-末端KEVSE的序列通过测序确定。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mM-CSF. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mM-CSF通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mM-CSF was determined in an M-NFS-60 cell proliferation assay. The ED50 of each lot is between 0.5-2.0 ng/ml.

重组蛋白mM-CSF的生物活性是通过M-NFS-60细胞增值实验确定的。每个批次的ED50在0.5-2.0 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mM-CSF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mM-CSF and staining overnight with Coomassie Blue.重组蛋白mM-CSF 的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from M-NFS-60 cells, untreated or treated with mM-CSF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western免疫印迹。用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (上图)和p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (下图)研究未经处理和经过mM-CSF 处理10分钟的M-NFS-60 细胞的细胞提取液。

Bioactivity

Bioactivity

The proliferation of M-NFS-60 cells treated with increasing concentrations of mM-CSF was assessed. After 48 hour treatment with mM-CSF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用递增浓度的重组蛋白mM-CSF处理M-NFS-60细胞并观察其增值实验。用mM-CSF处理48小时后用四唑盐孵育并测定OD450 - OD650 数值。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mM-CSF.

内毒素含量:<0.01 ng /1 μg mM-CSF。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mM-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mM-CSF蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mM-CSF蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

Macrophage-colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts, and other cell types (1). M-CSF is required for growth and differentiation of monocytes and macrophages (1,2). M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production (1,2). M-CSF binds to its receptor CSF1R; downstream signaling involves PI3K/Akt, Erk, and Stats 1, 3, and 5 (1,3). An increase in M-CSF expression may contribute to cancer progression, and high plasma M-CSF levels are associated with rheumatoid arthritis (1,4,5).

GM-CSF 是通过活化的T细胞,NK细胞和巨噬细胞产生(1,2)。靶标细胞包括粒性白细胞,单核细胞前体和经过分化的一系列的骨髓细胞(1,3,4)。很多的靶标细胞需要GM-CSF才能生存。GM-CSF 诱导增殖,并参与造血细胞的分化成树状细胞,它在干细胞分化的信号通路上也是主要的因素之一。GM-CSF 激活骨髓细胞的功能,所以它连接天然免疫和获得性免疫并推进抗肿瘤免疫(5)。GM-CSF受体由GM-CSFRα和共有的 β链-βC 组成,这同时也被IL-3和 IL-5所利用(1)。GM-CSF的结合起始了 Jak2, Stat5 和 PI3K/Akt 信号通路(1)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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