Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-17A (mIL-17A) #5227

Il-17   il17a  

No. Size Price
5227LC 50 µg ( With Carrier ) ¥6,860.00 现货查询 购买询价
5227LF 50 µg ( Carrier Free ) ¥6,860.00 现货查询 购买询价
5227SC 10 µg ( With Carrier ) ¥2,321.00 现货查询 购买询价
5227SF 10 µg ( Carrier Free ) ¥2,321.00 现货查询 购买询价
5227 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-17A (mIL-17A) Thr22-Ala158 (Accession #NP_034682) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的小鼠重组蛋白 IL-17A (mIL-17A) Thr22-Ala158 (Accession #NP_034682)是从人的293细胞表达而来。

Molecular Characterization

Recombinant mIL-17A contains no "tags" and the nonglycosylated protein has a calculated MW of 15,377. DTT-reduced protein migrates as a 15-22 kDa polypeptide. Heterogeneity in SDS PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 28-36 kDa protein. The expected amino-terminal TVKAA of recombinant mIL-17A was verified by amino acid sequencing.

重组的mIL-17A蛋白没有标签,未糖基化的蛋白分子量据推算为15,377Da。DTT-还原的蛋白分子作为15-22 kDa的蛋白而迁移。在SDS-PAGE中具有异质性是由于糖基化。未还原的蛋白由胱氨酸连接形成同源二聚体而作为28-36 kDa 蛋白迁移。重组mIL-17A 蛋白的N-末端TVKAA的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-17A. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) 重组mIL-17A通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mIL-17A was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 0.4-1.4 ng/ml.

mIL-17A 的生物活性是通过诱导3T3 MEFs WT产生IL-6能力的实验来确定的。每个批次的ED50在0.4-1.4ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-17A was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-17A and staining overnight with Coomassie Blue.重组mIL-17A 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-17A 蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The production of mouse IL-6 by 3T3 MEFs WT cultured with increasing concentrations of mIL-17A was assessed. Media from cells incubated with mIL-17A for 24 hours was collected and assayed for mouse IL-6 by ELISA and the OD450-OD650 was determined.用递增浓度的mIL-17A 培养3T3 MEFs WT 使其产生IL-6 的实验。用mIL-17A培养细胞24小时后收集培养基并通过 ELISA 确定IL-6并测定 OD450-OD650 数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from 3T3 MEFs WT untreated or treated with mIL-17A for 10 minutes, using Phospho-IκB-α (Ser32) (14D4) Rabbit mAb #2859 (upper) and IκB-α (L35A5) Mouse mAb #4814 (lower).Western免疫印迹。用Phospho-IκB-α (Ser32) (14D4) Rabbit mAb #2859(上图)和IκB-α (L35A5) Mouse mAb #4814 (下图) 研究未经处理的和经mIL-17A 处理10 min的 3T3 MEFs WT 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1μg mIL-17A.

每微克mIL-17A内毒素含量小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-17A. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mIL-17A蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-17A蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-17A is a cystine-linked homodimeric pro-inflammatory cytokine produced by TH17 cells, a distinct CD4+ T cell lineage (1,2). IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNFα, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity (1,2). IL-17A is intimately involved in mucosal immunity against bacterial infections (1,3) and has a putative role in some autoimmune disorders (1,4). IL-17A effects appear to be exerted primarily through binding to the IL-17RA (5). IL-17A binding induces production of cytokines, chemokines and other proteins through activation of the ERK1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (3,4,6). Phosphorylation of some Jaks and Stats has been observed.

IL-17A 是通过胱氨酸连接的同型二聚体,通过独特的CD4+ T细胞系产生的促炎细胞因子(1,2)。IL-17A 刺激促炎因子IL-1β、TNF-α和 IL-6的产生。IL-17A 也能够诱导产生噬中性粒细胞趋化剂如IL-8、CXCL1和 CXCL6。这样就将天然免疫和获得性免疫连接起来(1,2)。IL-17A跟粘膜抵抗细菌侵染有密切的联系(1,3) 同时也在自体免疫混乱过程中有潜在的功能(1,4)。IL-17A 通过结合到 IL-17RA发挥作用(5)。IL-17A与IL-17RA的结合通过激活Erk1/2 MAP激酶, PI3K/Akt、p38和 NF-κB 信号通路诱导产生细胞因子、趋化因子和一些相关的蛋白(3,4, 6)。它也能够磷酸化一些 Jaks 和 Stats 家族的蛋白。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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