Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-22 (mIL-22) #5224

il-22   IL22   interleukin 22   mIL-22   mil22  

No. Size Price
5224LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
5224LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
5224SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5224SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5224 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-22 (mIL-22) Leu34-Val179 (Accession #NP_058667) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的小鼠重组蛋白IL-22 (mIL-22) Leu34-Val179 (Accession #NP_058667)是从人的293细胞表达而来。

Molecular Characterization

Recombinant mIL-22 contains no "tags" and the nonglycosylated protein has a calculated MW of 16,640. DTT-reduced and non-reduced protein migrate as 31 kDa polypeptides. Lower mobility and heterogeneity in SDS PAGE are due to glycosylation. The expected amino-terminal LPVNT of recombinant mIL-22 was verified by amino acid sequencing.

重组的mIL-22 蛋白在N-末端没有标签,未糖基化蛋白分子量据推算为16,640Da。DTT-还原的蛋白和未还原的蛋白作为31kDa的多肽而迁移。在SDS-PAGE中较低的迁移率是由于糖基化的作用。重组蛋白mIL-22 的N-末端序列LPVNT通过测序而证实。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-22. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) 重组hIL-22通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mIL-22 was determined by its ability to induce IL-10 production by COLO 205 cells. The ED50 of each lot is between 0.2-0.8 ng/ml.

mIL-22 的生物活性是通过诱导COLO 205细胞产生IL-10能力的实验来确定的。每个批次的ED50在 0.2-0.8 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-22 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-22 and staining overnight with Coomassie Blue.重组mIL-22蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-22蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The production of IL-10 by COLO 205 cells cultured with increasing concentrations of mIL-22 was assessed. Media from cells incubated with mIL-22 for 48 hours was collected and assayed for IL-10 by ELISA and the OD450-OD650 was determined.用浓度递增的mIL-22 诱导COLO 205细胞产生IL-10能力的实验。用mIL-22孵育细胞48小时后取上清,通过ELISA 和OD450-OD650 测定IL-10 的量。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, untreated or treated with mIL-22 for 15 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).Western免疫印迹。用 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (上图)和 Stat3 Antibody #9132 (下图) 研究未经处理的和经mIL-22 处理15分钟的HT-29 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1μg mIL-22.

每微克mIL-22内毒素含量小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-22. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体:每微克mIL-22蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-22蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-22, a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells and NK cells (3). Expression of IL-22 in combination with a select group of cytokines defines a subset of Th cells(4). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue-repair and wound-healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (5). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohn's disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (6).

IL-22, 是IL-10家族的一员 (1,2),Th17 CD4+ T 细胞、激活的 T细胞、Th1 细胞和NK 细胞都能表达IL-22(3)。IL-22与一组细胞因子一起表达,这组细胞定义为Th细胞(4)。IL-22诱导促炎症反应,促使抗细菌多肽的产生,同时也参与到组织修复和伤口愈合过程中(1)。IL-22的受体是 IL-22R1和IL-10R2异源二聚体(5)。IL-22R的表达局限于组织细胞中尤其是上皮起源的细胞中,然而IL-10R2链在更多的细胞类型中表达。IL-22 诱导Jak1和 Tyk2的磷酸化,激活Stat3,也在较低程度上可以激活Stat1和Stat5 (1)。IL-22 反应参与到MEK-ERK-RSK、JNK-SAPK和 p38 信号通路的激活过程中(1)。IL-22表达的升高与Crohn's 病和类风湿性关节炎有关系。IL-22 在宿主抵抗肺部细菌克雷伯菌的应答中发挥了重要的作用(6)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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