Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Stem Cell Factor (mSCF) #5223

No. Size Price
5223SC 10 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
5223SF 10 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
5223 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse SCF (mSCF) Lys26-Ala189 (Accession #NP_038626) was expressed in human 293 cells at Cell Signaling Technology.

Cell Signaling Technology使用人293细胞表达重组鼠SCF (mSCF) Lys26-Ala189 (Accession #NP_038626)。

Molecular Characterization

Recombinant mSCF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,298. DTT-reduced and non-reduced protein migrate as 22-35 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal KEICG of recombinant mSCF was verified by amino acid sequencing.

重组mTGF-β1蛋白不带标记且非糖基化的蛋白分子量为18,298。DTT-还原状态下蛋白迁移状态与22-35 KDa多肽相同。SDS-PAGE中的低迁移率和不均一性是由糖基化导致的。重组mSCF氨基端序列KEICG通过氨基酸测序确认。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mSCF. All lots are greater than 98% pure.

还原和未还原的mEGF由SDS-PAGE测定纯度大于98%。所有批次的产品纯度均大于98%。

Bioactivity

The bioactivity of recombinant mSCF was determined in an M-07e cell proliferation assay. The ED50 of each lot is between 2-8 ng/ml.

重组mSCF的生物活性通过M-07e细胞增殖实验而检测。每一批次的ED50均在2-8 ng/ml之间。

Bioactivity

Bioactivity

The proliferation of M-07e cells treated with increasing concentrations of mSCF was assessed. After 48 hour treatment with mSCF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.逐渐增加浓度的mSCF对M-07细胞的增殖进行实验验证。使用mSCF处理48小时候,用一种四唑盐孵育细胞,检测OD450 - OD650 。

Western Blotting

Western Blotting

Western blot analysis of extracts from M-07e cells untreated or treated with mSCF for 5 minutes, using Phospho-c-Kit (Tyr719) Antibody #3391 (upper) and c-Kit (D13A2) XP® Rabbit mAb #3074 (lower).mSCF处理M-07细胞5分钟,或未处理的细胞提取无进行Western blot分析,使用ho-c-Kit (Tyr719)抗体#3391(上)和c-Kit(D13A2) XP®兔源单克隆抗体#3074(下)进行检测。

Coomassie Gel

Coomassie Gel

The purity of recombinant mSCF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mSCF and staining overnight with Coomassie Blue.重组mSCF的纯度使用SDS-PAGE进行确认,6 ug还原(+)或未还原(-)的重组mSCF进行电泳后用考马斯蓝染色过夜。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mSCF.1ug mSCF蛋白所含内毒素低于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mSCF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

带载体:每1 μg mSCF溶于含有20 μgBSA、pH7.2的PBS溶液经0.22 μm滤器过滤后冻干。无载体:溶于pH7.2的PBS溶液经0.22 μm滤器过滤获得冻干物。

Background

SCF is produced by endothelial cells, fibroblasts, keratinocytes, gut epithelial cells, and tumor cells (1,2). SCF is critical for hematopoiesis and mast cell differentiation and has additional roles in survival and function of other cell types (1). Some tumor cell proliferation and invasiveness are promoted by SCF (3). Tumor-derived SCF appears to be involved in expansion of myeloid-derived suppressor cells which, in turn, limits proliferation of tumor-infiltrating T-cells (4). SCF may have additional roles in the tumor microenvironment (2). SCF is either soluble or an integral membrane protein and its form is dependant on variation in splicing or proteolytic release (1). SCF binds to the receptor tyrosine kinase c-Kit and induces activation of the Akt, Erk, JNK, and p38 pathways (5,6).

SCF存在于内皮细胞,成纤维细胞,角质化细胞,肠上皮细胞和癌细胞(1,2)。SCF对于造血作用和肥大细胞的分化非常重要,并且也在其他类型细胞的生存等调控中扮演角色(1)。一些肿瘤细胞的增殖和侵袭受SCF蛋白起始(3)。肿瘤来源的SCF似乎涉及骨髓来源的抑制细胞的增殖,这种抑制细胞会限制肿瘤-侵润-T细胞的扩增(4)。SCF在肿瘤微环境中也有其他作用(2)。SCF可以以可溶蛋白状态或嵌膜蛋白状态存在,它的存在形式决定于剪切或蛋白水解释放的不同异构体(1)。SCF可以与酪氨酸激酶受体c-Kit结合,随后激活Akt, Erk, JNK和p38 信号通路 (5,6)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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