Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interferon-γ (mIFN-γ) #5222

IFN   IFN gamma   IFN-gamma   ifng   interferon  

No. Size Price
5222SC 100 µg ( With Carrier ) ¥3,590.00 现货查询 购买询价 防伪查询
5222SF 100 µg ( Carrier Free ) ¥3,590.00 现货查询 购买询价 防伪查询
5222 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IFN-γ (mIFN-γ) His23-Cys155 (Accession # NP_032363) was produced in E. coli at Cell Signaling Technology.

CST公司生产的鼠源重组蛋白IFN-γ (mIFN-γ) His23-Cys155 (Accession # NP_032363)是从大肠杆菌细胞表达而来。

Molecular Characterization

Recombinant mIFN-γ has a Met on the amino terminus and has a calculated MW of 15,652. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminus MHGTV of recombinant mIFN-γ was verified by amino acid sequencing.

重组mIFN-γ蛋白在N-末端有Met标签,蛋白分子量据推算为15,652Da。DTT-还原蛋白和未还原蛋白作为14kDa的多肽迁移。重组mIFN-γ蛋白的N-末端MHGTV的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIFN-γ. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) 重组mIFN-γ通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of mIFN-γ was determined in a virus protection assay. The ED50 of each lot is between 30-150 pg/ml.

mIFN-γ的生物活性是通过病毒保护实验来确定的。每个批次的ED50在30-150 pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIFN-γ was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIFN-γ and staining overnight with Coomassie Blue.重组mIFN-γ蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIFN-γ蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The bioactivity of recombinant mIFN-γ was determined in a virus protection assay. L-929 cells were pretreated with increasing concentrations of mIFN-γ for 24 hours. Cells were then innoculated with encephalomyocarditis virus (EMCV) and incubated for an additional 48 hours. Surviving cells were then fixed and stained with crystal violet and the OD595 was determined.mIFN-γ的生物活性是通过病毒保护实验来确定的。用浓度递增的mIFN-γ预处理L-929 细胞24小时,然后放在脑心肌炎病毒中培养48小时,存活的细胞通过固定和结晶紫染色并测定OD595数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from L-929 cells, untreated or treated with mIFN-γ for 20 minutes, using Phospho-Stat1 (Tyr701) Antibody #9171 (upper) and Stat1 Antibody #9172 (lower).Western免疫印迹。用Phospho-Stat1 (Tyr701) Antibody #9171 (上图)和Stat1 Antibody #9172 (下图) 研究未经处理的和经mIFN-γ处理20 min的L-929 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIFN-γ.

每微克mIFN-γ内毒素含量小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIFN-γ. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体:每微克mIFN-γ蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIFN-γ蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen-presenting cells (APCs) promotes cell-mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increased class I and class II MHC expression, and enhancing peptide antigen presentation (1). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice, and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).

IFN-γ在天然免疫和获得性免疫应答中发挥重要作用。IFN-γ激活天然免疫细胞如巨噬细胞和NK细胞的细胞毒活性(1,2)。通过NK细胞和具有抗原细胞(APCs)产生IFN-γ促进了细胞介导的获得性免疫,包括诱导T淋巴细胞产生IFN-γ、增加I型和II型 MHC 分子的表达、增强肽抗原呈递(1)。IFN-γ的抗病毒活性与PKR蛋白的诱导和其它的调控蛋白有关。IFN-γ结合到 IFNGR1/IFNGR2 复合体促进了受体的二聚化并形成(IFNGR1/IFNGR2)2 -IFN-γ二聚体。这种结合诱导了受体胞内域构象的改变并激活包括Jak1、 Jak2和 Stat1 的信号通路(3)。IFN-γ的重要作用是放大免疫监控,在IFN-γ或IFNGR敲除老鼠和IFNGR1或IFNGR2失活突变体中,病原菌感染的易感性增加。IFN-γ在动脉硬化中也发挥了重要的作用(4)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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