Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human β-Nerve Growth Factor (hβ-NGF) #5221

βNGF  

No. Size Price
5221LC 50 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5221LF 50 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5221SC 10 µg ( With Carrier ) ¥1,302.00 现货查询 购买询价
5221SF 10 µg ( Carrier Free ) ¥1,302.00 现货查询 购买询价
5221 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human β-NGF (hβ-NGF) Ser122-Ala241 (Accession #NP_002497) was expressed in human 293 cells at Cell Signaling Technology.

CST公司在人293细胞中生产重组人蛋白β-NGF (hβ-NGF) Ser122-Ala241 (Accession #NP_002497)。

Molecular Characterization

Based on amino acid sequencing, greater than 85% of recombinant hβ-NGF starts at Ser122 (SSSHP) and has a calculated MW of 13,494. The remainder starts at Ser124 (SHPIF). DTT-reduced and non-reduced protein migrate as 13 kDa non-disulfide linked homodimers.

基于氨基酸测序结果,85%以上的重组hβ-NGF 蛋白在 Ser122 (SSSHP)位点起始,据推算分子量为 13,494Da。其余的蛋白在Ser124 (SHPIF)位点起始。DTT-还原和未还原蛋白作为13kDa的非二硫键连接的二聚体多肽而转移。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hβ-NGF. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) hβ-NGF通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant hβ-NGF was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 0.5-1.5 ng/ml.

重组蛋白hβ-NGF 的生物活性是通过TF-1细胞的细胞增殖实验确定的。每个批次的ED50在0.5-1.5 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hβ-NGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hβ-NGF and staining overnight with Coomassie Blue.重组蛋白hβ-NGF 的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of TF-1 cells treated with increasing concentrations of hβ-NGF was assessed. After 48 hour treatment with hβ-NGF, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.用递增浓度的hβ-NGF处理TF-1 并验证增殖的实验。用hβ-NGF处理48小时后,细胞与四唑盐孵育,然后测定OD450 - OD650数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from TF-1 cells untreated or treated with hβ-NGF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western免疫印迹。用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (上图)和p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695(下图)研究未经处理和经hβ-NGF 处理10分钟的TF-1细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hβ-NGF.

内毒素含量:<0.01 ng /1 μg hβ-NGF。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hβ-NGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克hβ-NGF蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克hβ-NGF蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

β-NGF is the prototypical member of the neurotrophin family of growth factors (1). β-NGF is involved in neuronal survival, differentiation and growth (1,2). Outside of the nervous system, NGF is produced by a variety of immune cells, including B cells, T cells, monocytes and mast cells (3,4). β-NGF binds to and signals through two distinct receptors, TrkA and p75NTR (1,2). Cellular responses induced by NGF are modulated by receptor expression. For example, TrKA leads to the inhibition of apoptosis and neuronal differentiation. In contrast, signaling through p75NTR in the absence of TrkA induces cell death. (1,2). NGF signaling via TrkA is characterized by activation of the PI3K/AKT and PLCγ pathways (1,2). NGF signaling via p75NTR induces JNK and NFκB activation (1,2). Aberrant NGF signaling may be linked to the onset of Alzheimer’s disease (5,6).

β-NGF是生长因子神经营养因子的经典成员之一(1)。β-NGF参与到神经元的存活、分化和生长(1,2)。在神经系统外围, NGF通过一系列的免疫细胞产生,包括B细胞、T细胞、单核细胞核柱状细胞(3,4)。β-NGF结合到两个不同的受体并TrkA和p75NTR上并引起信号(1,2)。NGF诱导的胞内响应通过受体表达而得到调节。例如, TrKA导致了凋亡的抑制和神经的分化。相反, 通过p75NTR的信号通路(在 TrkA不存在时)诱导细胞死亡(1,2)。NGF通过TrkA的信号的特点是对PI3K/AKT和PLCγ信号通路的激活(1,2)。NGF通过p75NTR的信号诱导 JNK和NFκB 的激活(1,2)。异常的NGF信号可能与阿尔茨海默病的发病有关(5,6)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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