Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-7 (mIL-7) #5217

il-7   il7   Interleukin 7   mIL7  

No. Size Price
5217LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
5217LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
5217SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5217SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5217 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-7 (mIL-7) Glu26-Ile154 (Accession #NP_032397) was produced in E. coli at Cell Signaling Technology.

CST公司在大肠杆菌中生产重组鼠蛋白IL-7 (mIL-7) Glu26-Ile154 (Accession #NP_032397)。

Molecular Characterization

Recombinant mIL-7 has a Met on the amino terminus and has a calculated MW of 15,028. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides. The expected amino-terminal MECHI of recombinant mIL-7 was verified by amino acid sequencing.

重组的hIL-8蛋白在N-末端有Met标签,分子量据推算为15,028Da。DTT-还原和未还原的蛋白迁移时是作为一个14kDa 的多肽而转移,重组mIL-7蛋白的N-末端MECHI的序列通过测序确定。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-7. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mIL-7通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mIL-7 was determined in a 2E8 cell proliferation assay. The ED50 of each lot is between 60-200 pg/ml.

重组蛋白mIL-7的生物活性是通过2E8细胞增值实验确定的。每个批次的ED50在60-200 pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-7 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-7 and staining overnight with Coomassie Blue.重组蛋白mIL-7的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of 2E8 cells treated with increasing concentrations of mIL-7 was assessed. After 48 hour treatment withmIL-7, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用浓度递增重组蛋白mIL-7对2E8细胞进行处理观察其增值实验。2E8处理48小时后,细胞在四唑盐中孵育并测定OD450 - OD650 数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from 2E8 cells, untreated or treated with mIL-7 for 15 minutes, using Phospho-Stat5 (Tyr695) (C11C5) Rabbit mAb Antibody #9359 (upper) and Stat5 (3H7) Antibody #9358 (lower).Western免疫印迹。用Phospho-Stat5 (Tyr695) (C11C5) Rabbit mAb Antibody #9359(上图)和Stat5 (3H7) Antibody #9358 (下图)研究未经处理和经过mIL-7处理15分钟的2E8细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-7.

内毒素含量:<0.01 ng /1 μg mIL-7。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-7. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mIL-7蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-7蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-7 plays key a role in lymphopoiesis, and lymphoid homeostasis (1). Stromal and epithelial cells within the bone marrow and thymus produce IL-7 (1). The primary targets of IL-7 are T cells, B cells, and dendritic cells (1). IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling (1,2). While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear (1,3). In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of naïve and memory α/β T cells as well as γδ T cells (1). The IL-7 receptor is a heterodimer of the common γ chain, γc, and the IL-7 specific IL-7Rα(1). IL-7 activates PI3K/Akt, Jak 1/2, and Stat 1, 3, and 5 (1).

IL-7 在淋巴细胞增殖和淋巴细胞的动态平衡中发挥了重要的作用(1)。在骨髓和胸腺中的基质和上皮细胞产生IL-7(1)。IL-7最主要的靶标是T细胞、B细胞和树突细胞(1)。IL-7对T细胞的发育非常重要,此重要性在缺失T细胞的小鼠和人中的IL-7/IL-7R信号缺陷中被低估(1,2)。同时,在小鼠中IL-7是B细胞的发育中必需的, IL-7在人的B细胞发育中的作用尚不清楚(1,3)。除了它在T淋巴细胞增殖中的影响, IL-7促进空白和记忆αβ T和γδ T细胞的维持和存活(1)。IL-7的受体是共有的γ链-γc和 IL-7特异的IL-7Rα构成的异质二聚体(1)。IL-7激活PI3K/Akt, Jak1/2 和 Stat1, 3和 5 (1)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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