Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-6 (mIL-6) #5216

No. Size Price
5216LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
5216LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
5216SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5216SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5216 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-6 (mIL-6) Phe25-Thr211 (Accession #NP_112445) was expressed in human 293 cells at Cell Signaling Technology.

CST公司利用重组的鼠IL-6 (mIL-6) Phe25-Thr211 (Accession #NP_112445)在人的293细胞中表达。

Molecular Characterization

Recombinant mIL-6 contains no "tags" and the nonglycosylated protein has a calculated MW of 21,734. DTT-reduced and non-reduced protein migrate as 31 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation. The expected amino-terminal FPTSQ of recombinant mIL-6 was verified by amino acid sequencing.

重组的mIL-6蛋白在末端没有标签,未糖基化的蛋白分子量据推算为21,734Da。DTT-还原和未还原的蛋白迁移时是作为一个31kDa 的多肽而转移。其在SDS-PAGE胶中的低迁移性质是由于糖基化。重组mIL-6蛋白的N-末端序列FPTSQ通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-6. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mIL-6通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mIL-6 was determined in a B9 cell proliferation assay. The ED50 of each lot is between 0.5 and 2 pg/ml.

重组蛋白mIL-6的生物活性是通过B9细胞增殖实验确定的。每个批次的ED50在0.5-2pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-6 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-6 and staining overnight with Coomassie Blue.重组蛋白mIL-6 的纯度用6 µg 还原 (+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of B9 cells treated with increasing concentrations of mIL-6 was assessed. After 48 hour treatment with mIL-6, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用逐渐递增浓度的mIL-6处理B9细胞的细胞增殖实验。在mIL-6中处理48小时,细胞在四唑盐中孵育并读取OD450 - OD650 的数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from B9 cells, untreated or treated with increasing concentrations of mIL-6 for 10 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) and Stat3 (79D7) Rabbit mAb #4904 (lower).Western免疫印迹。用Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145(上图)和Stat3 (79D7) Rabbit mAb #4904(下图)研究未经处理和用递增浓度mIL-6处理10分钟的B9细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-6.

内毒素含量:<0.01 ng/1 μg mIL-6。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-6. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mIL-6蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-6蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-6 is a potent inducer of the acute phase response and is produced by T cells, macrophages, fibroblasts, endothelial, and other cells (1,2). IL-6 induces proliferation and differentiation and acts on B cells, T cells, thymocytes, and others. IL-6 in concert with TGFβ is important for developing Th17 responses. IL-6 binds to IL-6Rα and through this association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak/Stat cascade and the SHP2/MAPK (Erk) cascade (1,3,4). IL-6 also forms a complex with an IL-6Rα splice variant that is non-membrane associated (3).The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway on cells that express gp130 but not IL-6Rα (3). IL-6, through increasing expression of proangiogenic VEGF, may contribute to metastatic breast cancer (5).

IL-6是一个潜在的急性期应答的诱导因子,它由T细胞、巨噬细胞、纤维原细胞、内皮细胞和其它细胞产生(1,2)。IL-6诱导细胞增殖和分化并作用于B细胞、T细胞、胸腺细胞和其它细胞之中。IL-6与TGF-β相作用对Th17应答的发展非常重要。IL-6与IL-6Rα结合并通过此结合诱导gp130的同型二聚化(1)。gp130的同型二聚化引起Jak/Stat级联反应和SHP2/MAPK (Erk)级联反应(1,3,4)。IL-6也能与IL-6Rα可变剪切形成与膜无关的复合体(3)。IL-6/可溶性 IL-6Rα 复合体在表达gp130的细胞上能激活gp130信号通路,但不是IL-6Rα (3)。一些研究证明IL-6可能通过增加促血管新生因子 VEGF对乳腺癌的转移有作用(5)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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