Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-4 (mIL-4) #5208

il-4   interleukin 4   mIL-4  

No. Size Price
5208SC 10 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
5208SF 10 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
5208 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-4 (mIL-4) His21-Ser140 (Accession #NP_067258) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的鼠源重组蛋白IL-4 (mIL-4) His21-Ser140 (Accession #NP_067258)是从人源293细胞表达而来。

Molecular Characterization

Recombinant mIL-4 contains no "tags" and the nonglycosylated protein has a calculated MW of 13,557. DTT-reduced and non-reduced protein migrate as 16 kDa polypeptide due to glycosylation. The expected amino-terminal HIHGC of recombinant mIL-4 was verified by amino acid sequencing.



>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-4. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mIL-4通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。


The bioactivity of recombinant mIL-4 was determined in an HT-2 cell proliferation assay. The ED50 of each lot is between 100-400 pg/ml.

重组蛋白mIL-4的生物活性是通过HT-2细胞的增殖实验确定的。每个批次的ED50值在100-400 pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-4 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-4 and staining overnight with Coomassie Blue.重组mIL-4 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-4蛋白跑SDS胶并用考马斯亮蓝染色过夜。



The proliferation of HT-2 cells treated with increasing concentrations of mIL-4 was assessed. After 48 hour treatment with mIL-4, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在mIL-4蛋白浓度递增条件下研究HT-2细胞增殖实验。用mIL-4培养细胞48小时后与四唑盐孵育。并测定OD450 - OD650值。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-2 cells untreated or treated with mIL-4 for 10 minutes, using (upper) or(lower).Western免疫印迹。用Phospho-Jak1 (Tyr1022/1023) Antibody #3331 (上图) 和 Jak1(6G4) Rabbit mAb #3344 (下图) 研究未经处理的和经mIL-4处理10 min的HT-2细胞的细胞提取液。


Less than 0.01 ng endotoxin/1 μg mIL-4.

内毒素含量:小于0.01 ng /1 μg mIL-4。


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-4. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mIL-4蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-4蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。


IL-4 is produced by T cells, NK T cells, γδ cells, and mast cells (1). Target cells include B cells, T cells, and macrophages (1). IL-4 induces the polarization of naïve helper T cells into the TH2 phenotype (1,2). IL-4 also promotes B cell proliferation, antibody class switching and the production other TH2 cytokines including IL-5 and IL-9. IL-4 induced TH2 polarization is important in developing humoral immunity against extracellular pathogens (1) and is involved in the development of allergy and asthma (3). IL-4 binds to two distinct receptors, the Type I receptor and Type II receptor. Type I receptor is a heterodimer consisting of IL-4Rα chain and the common gamma chain, γc (4,5). Type II receptor, which is shared with IL-13, is a heterodimer of IL-4Rα and IL-13Rα1. Signaling initiated via Type I receptor results in the activation of Jak1/Stat6, Jak3 and the PI3K/Akt pathways (4). The Type II receptor activates the Jak1/Stat6 and the Tyk2/Stat3 pathways (4).

IL-4通过T 细胞、NK T 细胞、γδ 细胞和肌肉细胞产生(1)。目标细胞包括B 细胞、T 细胞和巨噬细胞(1)。IL-4 诱导T助细胞极化为TH2型。IL-4也能促进B细胞增殖、抗体分泌型转换和其它TH2细胞因子IL-5和IL-9细胞因子的表达。IL-4诱导TH2的极化,对病原菌体液免疫的发展有重要的作用(1),同时也参与到敏感症和哮喘的发病过程中(3)。IL-4 结合到两个不同的受体上, type I 受体和type II 受体。Type I 受体是异源二聚体包含IL-4Rα 链和普遍存在的 γ链-γc (4,5)。Type II 受体, 与IL-13受体共享, 是IL-4Rα 和 IL-13Rα1的二聚体。通过type I受体进行传递的信号能激活 Jak1/Stat6, Jak3 和 PI3K/Akt通路 (4)。type II 受体能激活 Jak1/Stat6和Tyk2/Stat3通路(4)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :