Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-4 (mIL-4) #5208

il-4   interleukin 4   mIL-4  

No. Size Price
5208LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
5208LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
5208SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5208SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5208 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-4 (mIL-4) His21-Ser140 (Accession #NP_067258) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的鼠源重组蛋白IL-4 (mIL-4) His21-Ser140 (Accession #NP_067258)是从人源293细胞表达而来。

Molecular Characterization

Recombinant mIL-4 contains no "tags" and the nonglycosylated protein has a calculated MW of 13,557. DTT-reduced and non-reduced protein migrate as 16 kDa polypeptide due to glycosylation. The expected amino-terminal HIHGC of recombinant mIL-4 was verified by amino acid sequencing.

重组的mIL-4蛋白在N-末端没有标签,未糖基化的蛋白分子量据推算为13,557Da。DTT-还原蛋白和未还原蛋白由于糖基化的存在而作为一个16kDa的蛋白转移。重组mIL-4蛋白的N-末端HIHGC的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-4. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mIL-4通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mIL-4 was determined in an HT-2 cell proliferation assay. The ED50 of each lot is between 100-400 pg/ml.

重组蛋白mIL-4的生物活性是通过HT-2细胞的增殖实验确定的。每个批次的ED50值在100-400 pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-4 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-4 and staining overnight with Coomassie Blue.重组mIL-4 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-4蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of HT-2 cells treated with increasing concentrations of mIL-4 was assessed. After 48 hour treatment with mIL-4, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在mIL-4蛋白浓度递增条件下研究HT-2细胞增殖实验。用mIL-4培养细胞48小时后与四唑盐孵育。并测定OD450 - OD650值。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-2 cells untreated or treated with mIL-4 for 10 minutes, using (upper) or(lower).Western免疫印迹。用Phospho-Jak1 (Tyr1022/1023) Antibody #3331 (上图) 和 Jak1(6G4) Rabbit mAb #3344 (下图) 研究未经处理的和经mIL-4处理10 min的HT-2细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-4.

内毒素含量:小于0.01 ng /1 μg mIL-4。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-4. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mIL-4蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-4蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-4 is produced by T cells, NK T cells, γδ cells, and mast cells (1). Target cells include B cells, T cells, and macrophages (1). IL-4 induces the polarization of naïve helper T cells into the TH2 phenotype (1,2). IL-4 also promotes B cell proliferation, antibody class switching and the production other TH2 cytokines including IL-5 and IL-9. IL-4 induced TH2 polarization is important in developing humoral immunity against extracellular pathogens (1) and is involved in the development of allergy and asthma (3). IL-4 binds to two distinct receptors, the Type I receptor and Type II receptor. Type I receptor is a heterodimer consisting of IL-4Rα chain and the common gamma chain, γc (4,5). Type II receptor, which is shared with IL-13, is a heterodimer of IL-4Rα and IL-13Rα1. Signaling initiated via Type I receptor results in the activation of Jak1/Stat6, Jak3 and the PI3K/Akt pathways (4). The Type II receptor activates the Jak1/Stat6 and the Tyk2/Stat3 pathways (4).

IL-4通过T 细胞、NK T 细胞、γδ 细胞和肌肉细胞产生(1)。目标细胞包括B 细胞、T 细胞和巨噬细胞(1)。IL-4 诱导T助细胞极化为TH2型。IL-4也能促进B细胞增殖、抗体分泌型转换和其它TH2细胞因子IL-5和IL-9细胞因子的表达。IL-4诱导TH2的极化,对病原菌体液免疫的发展有重要的作用(1),同时也参与到敏感症和哮喘的发病过程中(3)。IL-4 结合到两个不同的受体上, type I 受体和type II 受体。Type I 受体是异源二聚体包含IL-4Rα 链和普遍存在的 γ链-γc (4,5)。Type II 受体, 与IL-13受体共享, 是IL-4Rα 和 IL-13Rα1的二聚体。通过type I受体进行传递的信号能激活 Jak1/Stat6, Jak3 和 PI3K/Akt通路 (4)。type II 受体能激活 Jak1/Stat6和Tyk2/Stat3通路(4)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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