Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-1β (mIL-1β) #5204

il-1   il-1b   IL-1F2   IL-1β   IL1-β   IL1β   interleukin-1   Interleukin-1-β   Interleukin-1β   interleukin1   Interleukin1β   mIL-1beta  

No. Size Price
5204LC 50 µg ( With Carrier ) ¥10,050.00 现货查询 购买询价
5204LF 50 µg ( Carrier Free ) ¥10,050.00 现货查询 购买询价
5204SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5204SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5204 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-1β (mIL-1β) Val118-Ser269 (Accession #NP_032387) was produced in E.coli at Cell Signaling Technology.

CST公司生产的鼠源重组蛋白IL-1β (mIL-1β) Val118-Ser269 (Accession #NP_032387)是从大肠杆菌细胞表达而来。

Molecular Characterization

Recombinant mIL-1β has a Met on the amino terminus and has a calculated MW of 17,525. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal MVPIR of recombinant mIL-1β was verified by amino acid sequencing.

重组的 mIL-1β 蛋白在N-末端有Met标签,其蛋白分子量据推算为17,525Da。DTT-还原和未还原的蛋白作为一个18kDa的蛋白转移。重组mIL-1β蛋白的N-末端MVPIR的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-1β. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mIL-1β通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mIL-1β was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 5-20 pg/ml.

重组的mIL-1β生物活性是通过诱导3T3 MEFs WT细胞产生IL-6的能力来确定的。每个批次的ED50在5-20 pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-1β was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-1β and staining overnight with Coomassie Blue.重组mIL-1β 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-1β 蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from 3T3 MEFs WT untreated or treated with mIL-1β for 15 minutes, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 (E498) Antibody #3987 (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (上图)和NF-κB p65 (E498) Antibody #3987 (下图) 研究未经处理和经mIL-1β 处理15min的3T3 MEFs WT 细胞的细胞提取液。

Bioactivity

Bioactivity

The production of mouse IL-6 by 3T3 MEFs WT cultured with increasing concentrations of mIL-1β was assessed. Media from cells incubated with mIL-1β for 24 hours was collected and assayed for mouse IL-6 by ELISA and the OD450-OD650 was determined.用浓度递增mIL-1β 蛋白培养3T3 MEFs WT 细胞并研究其产生IL-6能力实验。用mIL-1β 培养细胞24小时后取培养基经ELISA测定IL-6并测定OD450 - OD650值。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-1β.

每微克mIL-1β内毒素含量小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-1β. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mIL-1β蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-1β蛋白在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-1β is a pro-inflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).

IL-1β是促炎症因子,主要的由激活的单核细胞和上皮细胞产生(1)。IL-1β的前体被caspase-1剪切,成熟的IL-1β紧接着被分泌(1-3)。目标细胞包括巨噬细胞和许多其它的细胞类型。IL-1β引起的信号通路包括IL-1β结合到IL-1的附属蛋白 (IL-1-AcP); 此复合体紧接着结合到IL-1RI (1,2)。信号通过激活MAP激酶和 NF-κB信号通路(1,2)。IL-1β也结合到IL-1RII,它缺乏细胞内的信号区域并因此作为高亲和性的诱捕受体。IL-1β结合到IL-1RI的过程能够被负调控因子IL-1R拮抗剂IL-1Ra所抑制。IL-1Ra结合到IL-1RI后无信号传导并且作为抑制剂阻止IL-1β信号通路。IL-1β在急性应答期和脓毒病中发挥了重要的作用(1-3)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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