Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-1β (mIL-1β) #5204

il-1   il-1b   IL-1F2   IL-1β   IL1-β   IL1β   interleukin-1   Interleukin-1-β   Interleukin-1β   interleukin1   Interleukin1β   mIL-1beta  

No. Size Price
5204SC 10 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
5204SF 10 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
5204 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-1β (mIL-1β) Val118-Ser269 (Accession #NP_032387) was produced in E.coli at Cell Signaling Technology.

CST公司生产的鼠源重组蛋白IL-1β (mIL-1β) Val118-Ser269 (Accession #NP_032387)是从大肠杆菌细胞表达而来。

Molecular Characterization

Recombinant mIL-1β has a Met on the amino terminus and has a calculated MW of 17,525. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal MVPIR of recombinant mIL-1β was verified by amino acid sequencing.

重组的 mIL-1β 蛋白在N-末端有Met标签,其蛋白分子量据推算为17,525Da。DTT-还原和未还原的蛋白作为一个18kDa的蛋白转移。重组mIL-1β蛋白的N-末端MVPIR的序列通过测序得到。


>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-1β. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mIL-1β通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。


The bioactivity of recombinant mIL-1β was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 5-20 pg/ml.

重组的mIL-1β生物活性是通过诱导3T3 MEFs WT细胞产生IL-6的能力来确定的。每个批次的ED50在5-20 pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-1β was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-1β and staining overnight with Coomassie Blue.重组mIL-1β 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-1β 蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from 3T3 MEFs WT untreated or treated with mIL-1β for 15 minutes, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 (E498) Antibody #3987 (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (上图)和NF-κB p65 (E498) Antibody #3987 (下图) 研究未经处理和经mIL-1β 处理15min的3T3 MEFs WT 细胞的细胞提取液。



The production of mouse IL-6 by 3T3 MEFs WT cultured with increasing concentrations of mIL-1β was assessed. Media from cells incubated with mIL-1β for 24 hours was collected and assayed for mouse IL-6 by ELISA and the OD450-OD650 was determined.用浓度递增mIL-1β 蛋白培养3T3 MEFs WT 细胞并研究其产生IL-6能力实验。用mIL-1β 培养细胞24小时后取培养基经ELISA测定IL-6并测定OD450 - OD650值。


Less than 0.01 ng endotoxin/1 μg mIL-1β.

每微克mIL-1β内毒素含量小于0.01 ng。


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mIL-1β. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mIL-1β蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-1β蛋白在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。


IL-1β is a pro-inflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).

IL-1β是促炎症因子,主要的由激活的单核细胞和上皮细胞产生(1)。IL-1β的前体被caspase-1剪切,成熟的IL-1β紧接着被分泌(1-3)。目标细胞包括巨噬细胞和许多其它的细胞类型。IL-1β引起的信号通路包括IL-1β结合到IL-1的附属蛋白 (IL-1-AcP); 此复合体紧接着结合到IL-1RI (1,2)。信号通过激活MAP激酶和 NF-κB信号通路(1,2)。IL-1β也结合到IL-1RII,它缺乏细胞内的信号区域并因此作为高亲和性的诱捕受体。IL-1β结合到IL-1RI的过程能够被负调控因子IL-1R拮抗剂IL-1Ra所抑制。IL-1Ra结合到IL-1RI后无信号传导并且作为抑制剂阻止IL-1β信号通路。IL-1β在急性应答期和脓毒病中发挥了重要的作用(1-3)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :