Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Interleukin-2 (mIL-2) #5201

il-2   IL2   T cell growth factor   TCGF  

No. Size Price
5201LC 50 µg ( With Carrier ) ¥6,860.00 现货查询 购买询价
5201LF 50 µg ( Carrier Free ) ¥6,860.00 现货查询 购买询价
5201SC 10 µg ( With Carrier ) ¥2,321.00 现货查询 购买询价
5201SF 10 µg ( Carrier Free ) ¥2,321.00 现货查询 购买询价
5201 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse IL-2 (mIL-2) Ala21-Gln169 (Accession #NP_032392) was produced in E. coli at Cell Signaling Technology.

CST公司在大肠杆菌中生产重组鼠蛋白IL-2 (mIL-2) Ala21-Gln169 (Accession #NP_032392)。

Molecular Characterization

Based on amino acid sequencing, greater than 50% of recombinant mIL-2 has a Met on the amino-terminal Ala21 (MAPTS) and has a calculated MW of 17,231. The remainder starts at Ala21 (APTSS) or Pro22 (PTSSP). DTT-reduced and non-reduced protein migrate as 19 kDa polypeptides.

基于氨基酸测序结果,50%以上的重组mIL-2蛋白在N-末端具有Met标签Ala21 (MAPTS),据推算分子量为17,231Da。其余的蛋白在Ala21 (APTSS)或者Pro22 (PTSSP)位点起始。DTT-还原和未还原蛋白作为19kDa多肽而迁移。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-2. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mIL-2通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mIL-2 was determined in a CTLL-2 cell proliferation assay. The ED50 of each lot is between 0.5-2 ng/ml.

重组蛋白mIL-2的生物活性是通过CTLL-2细胞增值实验确定的。每个批次的ED50在0.5-2 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mIL-2 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-2 and staining overnight with Coomassie Blue.重组蛋白mIL-2的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。

Western Blotting

Western Blotting

Western blot analysis of extracts from CTLL-2 cells untreated or treated with mIL-2 for 10 min, using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (upper) and Stat5 (3H7) Rabbit mAb #9358 (lower).Western免疫印迹。用Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (上图)和Stat5 (3H7) Rabbit mAb #9358 (下图)研究未经处理和经过mIL-2处理10分钟的CTLL-2细胞的细胞提取液。

Bioactivity

Bioactivity

The proliferation of CTLL-2 cells treated with increasing concentrations of mIL-2 was assessed. After 48 hours treatment with mIL-2, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用浓度递增重组蛋白mIL-2 对CTLL-2 细胞进行处理观察其增值实验。mIL-2处理48小时后,细胞在四唑盐中孵育并测定OD450 - OD650 数值。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mIL-2.

内毒素含量:<0.01 ng /1 μg mIL-2。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM DTT and 20 μg BSA per 1 μg mIL-2. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 1 mM DTT.

有载体: 每微克mIL-2蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIL-2蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-2 is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of Treg cells (3,4). Conversely, IL-2 is involved in activation induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-2Rβ is also used by IL-15 (1,2). The common γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).

IL-2是T细胞刺激细胞因子,因其对T细胞和NK细胞增殖的诱导和激活而知名(1,2)。IL-2也能促进外周Treg细胞的发育(3,4)。相反的,IL-2也参与到了激活对细胞死亡(AICD)的诱导中,此过程在后T细胞的扩充以增加 CD4+ T 细胞的Fas水平(5)。IL-2的效应是通过一个三聚体受体而介导,此三聚体包含IL-2Rα, IL-2Rβ和共有的γ链-γc (1,2)。 IL-2Rα特异性的以较低亲和度与 IL-2结合,而包括IL-2Rβ和γc亚单元的受体复合体结合亲和度明显提高。IL-15 也能结合到IL-2Rβ上 (1,2)。共有的γc链也被其它的细胞因子所利用,包括IL-4, IL-7, IL-9, IL-15和IL-21(1,2)。IL-2的结合起始了信号的级联反应,包括 Jak1, Jak3, Stat5和PI3K/Akt信号通路(1,2)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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