Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Granulocyte Macrophage Colony Stimulating Factor (mGM-CSF) #5191

No. Size Price
5191SC 10 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
5191SF 10 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
5191 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse GM-CSF (mGM-CSF) Ala18-Lys141 (Accession #NP_034099) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的鼠源重组蛋白GM-CSF (mGM-CSF) Ala18-Lys141 (Accession #NP_034099)是从人源293细胞表达而来。

Molecular Characterization

Recombinant mGM-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 14,112.1. DTT-reduced protein migrates as a 22 kDa polypeptide and non-reduced protein migrates as a 19 kDa polypeptide due to intramolecular cystines. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal APTRS of recombinant mGM-CSF was verified by amino acid sequencing.

重组的mGM-CSF蛋白在N-末端没有标签,蛋白分子量据推算为14,112.1Da。DTT-还原蛋白作为一个22kDa的蛋白转移。未还原蛋白由于有分子内的胱氨酸存在而作为19 kDa 迁移。在SDS-PAGE中稍低的迁移率和异质性是由于糖基化。重组mGM-CSF 蛋白的N-末端APTRS的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mGM-CSF. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mGM-CSF通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mGM-CSF was determined in a MC/9 cell proliferation assay. The ED50 of each lot is between 6-25 pg/ml.

mGM-CSF的生物活性是通过 MC/9细胞增殖来确定的。每个批次的ED50在6-25pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mGM-CSF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mGM-CSF and staining overnight with Coomassie Blue.重组mGM-CSF蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mGM-CSF 蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The proliferation of MC/9 cells treated with increasing concentrations of mGM-CSF was assessed. After 48 hour treatment with mGM-CSF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在mGM-CSF 蛋白浓度递增条件下研究MC/9细胞增殖实验。用mGM-CSF培养细胞48小时后用四唑盐处理。并测定OD450 - OD650值。

Western Blotting

Western Blotting

Western blot analysis of extracts from MC/9 cells untreated or treated with mGM-CSF for 10 minutes, using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (upper) and Stat5 (3H7) Rabbit mAb #9358 (lower).Western免疫印迹。用Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (上图)和Stat5 (3H7) Rabbit mAb #9358 (下图) 研究未经处理的和经mGM-CSF 处理10 min的MC/9 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1μg mGM-CSF.

内毒素含量:小于0.01 ng /1 μg mGM-CSF。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mGM-CSF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mGM-CSF蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mGM-CSF蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

GM-CSF is produced by activated T cells, NK cells and macrophages (1,2). Target cells include granulocytes, monocyte precursors and subsets of differentiated myeloid cells (1,3,4). Many target cells require GM-CSF for survival. GM-CSF induces proliferation, is involved in the hematopoietic differentiation of dendritic cells and is a key factor in differentiation pathways leading from stem cells. GM-CSF activates effector functions of myeloid cells, thereby linking adaptive and innate immunity and in turn may boost anti-tumor immunity (5). GM-CSF receptor is composed of GM-CSFRα and the common β chain, βC, which is also utilized by IL-3 and IL-5 (1). Binding of GM-CSF initiates the Jak2, Stat5 and PI3K/Akt pathways (1).

GM-CSF 是通过活化的T细胞,NK细胞和巨噬细胞产生(1,2)。靶标细胞包括粒性白细胞,单核细胞前体和经过分化的一系列的骨髓细胞(1,3,4)。很多的靶标细胞需要GM-CSF才能生存。GM-CSF 诱导增殖,并参与造血细胞的分化成树状细胞,它在干细胞分化的信号通路上也是主要的因素之一。GM-CSF 激活骨髓细胞的功能,所以它连接天然免疫和获得性免疫并推进抗肿瘤免疫(5)。GM-CSF受体由GM-CSFRα和共有的 β链-βC 组成,这同时也被IL-3和 IL-5所利用(1)。GM-CSF的结合起始了 Jak2, Stat5 和 PI3K/Akt 信号通路(1)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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