Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Interleukin-29 (hIL-29) #5183

hIL-29   IFN-lambda-1   IL-29   Interferon lambda-1  

No. Size Price
5183LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
5183LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
5183SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
5183SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
5183 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human IL-29 (hIL-29) Gly20-Thr200 (Accession #NP_742152) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的人重组蛋白IL-29 (hIL-29) Gly20-Thr200 (Accession #NP_742152)是从人的293细胞表达而来。

Molecular Characterization

Recombinant hIL-29 contains no "tags" and the nonglycosylated protein has a calculated MW of 20,018. DTT-reduced and non-reduced protein migrate as 31 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal GPVPT of recombinant hIL-29 was verified by amino acid sequencing.

重组的hIL-29蛋白没有标签,未糖基化蛋白分子量据推算为20,018Da。DTT-还原的蛋白和未还原的蛋白作为31kDa的多肽而迁移。在SDS-PAGE中较低的迁移率是由于糖基化的作用。重组蛋白hIL-29的N-末端序列GPVPT通过测序而证实。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-29. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) 重组hIL-29通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of hIL-29 was determined in a virus protection assay. The ED50 of each lot is between 0.40-1.45 ng/ml.

hIL-29的生物活性是通过病毒保护实验来确定的。每个批次的ED50在0.40-1.45 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hIL-29 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-29 and staining overnight with Coomassie Blue.重组hIL-29 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组hIL-29 蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The bioactivity of recombinant hIL-29 was determined in a virus protection assay. Hep G2 cells were pre-treated with increasing concentrations of hIL-29 for 24 hours. Cells were then innoculated with encephalomyocarditis virus (EMCV) and incubated for an additional 24 hours. Surviving cells were then fixed and stained with crystal violet and the OD595 was determined.hIL-29 的生物活性是通过病毒保护试验确定的。Hep G2 经过递增浓度的hIL-29预处理24小时,随后细胞与闹心肌炎病毒 (EMCV) 共孵育另外的24小时。存活的细胞固定后用结晶紫染色并测定OD595数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from RPMI 8226 cells untreated or treated with hIL-29 for 15 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).Western免疫印迹。用 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (上图)和 Stat3 Antibody #9132 (下图) 研究未经处理的和经hIL-29 处理15分钟的RPMI 8226 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1μg hIL-29.

每微克hIL-29内毒素含量小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-29. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体:每微克mIFN-γ蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIFN-γ蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-29 is a member of the IFNλ family of cytokines and shares many functions with the type I family of interferons (1,2). IL-29 is produced by monocytes and dendritic cells in response to viral infection and stimulation with toll-like receptor ligands (1-3). IL-29 is a potent antiviral cytokine. The antiviral activities of IL-29 include the upregulation of MHC Class I expression on the cell surface, and the expression of PKR, MxA, and 2’, 5'-OAS (1,2). The IL-29 receptor consists of IL-28Rα and IL-10Rβ is expressed on most non-hematopoietic cells (3). IL-29 binding induces the phosphorylation of Stats 1, 2 and 3 (1,3).

IL-29是干扰素λ细胞因子家族成员之一,并参与到I型干扰素家族的功能中(1,2)。IL-29在单核细胞和树突细胞对病毒感染的应答过程中而产生并且刺激Toll样受体的配体(1-3)。IL-29是潜在的抗病毒的活性的细胞因子。IL-29的抗病毒活性包括上调I类MHC在细胞膜上的表达、PKR、MxA和 2’, 5'-OAS 的表达(1,2)。IL-29的受体由 IL-28Rα和IL-10Rβ组成并在多数非造血功能的细胞上表达 (3)。IL-29的结合诱导了 Stats 1, 2 和 3的磷酸化(1,3)。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications