Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse Tumor Necrosis Factor-α (mTNF-α) #5178

tnf-a   TNFa   TNFSF2  

No. Size Price
5178SC 10 µg ( With Carrier ) ¥2,386.00 现货查询 购买询价 防伪查询
5178SF 10 µg ( Carrier Free ) ¥2,386.00 现货查询 购买询价 防伪查询
5178 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse TNF-α (mTNF-α) Leu80-Leu235 (Accession #NP_038721) was expressed in human 293 cells at Cell Signaling Technology.

CST公司在人293细胞中生产重组鼠蛋白TNF-α (mTNF-α) Leu80-Leu235 (Accession #NP_038721)。

Molecular Characterization

Based on amino acid sequencing, greater than 50% of recombinant mTNF-α starts at Leu80 (LRSSS) and has a calculated MW of 17,257. The remainder starts at Ser82 (SSSQN). DTT-reduced and non-reduced protein migrate as 16 kDa polypeptides.

基于氨基酸测序结果,50%以上的重组mTNF-α蛋白在Leu80 (LRSSS)位点起始,据推算分子量为17,257Da。其余的蛋白在Ser82 (SSSQN)位点起始。DTT-还原和未还原蛋白作为16kDa多肽而迁移。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mTNF-α. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mTNF-α通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant mTNF-α was determined in a L-929 cell viability assay. The ED50 of each lot is between 2-8 pg/ml.

重组蛋白mTNF-α的生物活性是通过L-929 细胞的生存能力实验确定的。每个批次的ED50在2-8 pg/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant mTNF-α was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mTNF-α and staining overnight with Coomassie Blue.重组蛋白mTNF-α的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The viability of L-929 cells treated with increasing concentrations of mTNF-α in the presence of 2 ng/ml actinomycin D was assessed. Cells were stained with crystal violet at the end of treatment and the OD595 was determined.重组蛋白mTNF-α的生物活性是通过在用2 ng/ml 放线菌素D存在下用递增浓度的mTNF-α处理L-929 细胞并观查其生存能力实验确定的。细胞经过结晶紫染色并测定OD595数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with mouse TNF-α for 20 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western免疫印迹。用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (上图)和 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695(下图)研究未经处理和经小鼠TNF-α处理20分钟的HeLa细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg mTNF-α.

内毒素含量:<0.01 ng /1 μg mTNF-α。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mTNF-α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mTNF-α蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mTNF-α蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, ERK (p44/42), p38 MAPK and NF-κB) promotes the survival of cells, while TNF-α mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).

TNF-α, TNF家族的典型成员, 是II型的同源三聚体膜蛋白(1,2)。细胞膜锚定的TNF-α通过金属蛋白酶TACE/ADAM17的剪切形成可溶性的同型三聚体(2)。膜锚定和可溶性两种形式都有生物活性。TNF-α可由很多种类的细胞产生包括T细胞、B细胞、巨噬细胞和NK细胞(1)。TNF-α的细胞内响应是通过与 TNF-R1 和 TNF-R2 两个受体互作而介导的并激活细胞存活和细胞凋亡两个截然相反地过程,这两个过程主要决定于细胞的类型和生物学环境。激活激酶的通路(包括JNK, Erk (p44/42), p38 MAPK 和 NF-κB) 促使细胞的存活,TNF-α介导的对caspase-8的激活导致了程序性的细胞死亡(1,2)。TNF-α在炎症反应和宿主抵抗细菌入侵的过程特别是结核分枝杆菌发挥了重要的调控作用(3)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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