Cell Signaling Technology

Product Pathways - Apoptosis

N-Myc (D4B2Y) Rabbit mAb #51705

No. Size Price
51705S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
51705 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 62 Rabbit IgG
IP 1:200
IHC-P 1:640
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

N-Myc (D4B2Y) Rabbit mAb recognizes endogenous levels of total N-Myc protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly38 of human N-Myc protein.

IP

IP

Immunoprecipitation of N-Myc from IMR-32 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is N-Myc (D4B2Y) Rabbit mAb. Western blot was performed using N-Myc (D4B2Y) Rabbit mAb. A confirmation specific secondary antibody was used to avoid cross reactivity with IgG.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded IMR-32 (left) and HeLa (right) cell pellets using N-Myc (D4B2Y) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human neuroblastoma using N-Myc (D4B2Y) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human testis using N-Myc (D4B2Y) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 IMR-32 cells and either 10 µl of N-Myc (D4B2Y) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human MIR17HG intron 1 primers, SimpleChIP® Human MDM2 Intron 2 Primers #90678, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using N-Myc (D4B2Y) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).

In humans the Myc family consists of 5 genes: c-Myc, N-Myc, L-Myc, R-Myc, and B-Myc. While c-Myc is expressed in many proliferating cells, N-Myc expression is very restricted, with highest levels during embryonic development and in the adult during B-cell development. The expression patterns and results from targeted deletion of N-Myc suggest that N-Myc plays an important role in tissue development and differentiation (5). In addition, amplification or overexpression of N-Myc has been found in human neuroblastomas and is associated with rapid progression and poor prognosis (6,7).

  1. Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
  2. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
  3. Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
  4. Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.
  5. Sawai, S. et al. (1993) Development 117, 1445-1455.
  6. Schwab, M. et al. (1984) Proc. Natl. Acad. Sci. USA 81, 4940-4944.
  7. Brodeur, G.M. et al. (1984) Science 224, 1121-1124.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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