Product Pathways - Apoptosis
N-Myc (D4B2Y) Rabbit mAb #51705
|51705S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|51705||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), ChIP=Chromatin IP,
Species predicted to react based on 100% sequence homology: Rat,
Specificity / Sensitivity
N-Myc (D4B2Y) Rabbit mAb recognizes endogenous levels of total N-Myc protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly38 of human N-Myc protein.
Immunoprecipitation of N-Myc from IMR-32 cell extracts. Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is N-Myc (D4B2Y) Rabbit mAb. Western blot was performed using N-Myc (D4B2Y) Rabbit mAb. A confirmation specific secondary antibody was used to avoid cross reactivity with IgG.
Immunohistochemical analysis of paraffin-embedded IMR-32 (left) and HeLa (right) cell pellets using N-Myc (D4B2Y) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human neuroblastoma using N-Myc (D4B2Y) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human testis using N-Myc (D4B2Y) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 IMR-32 cells and either 10 µl of N-Myc (D4B2Y) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human MIR17HG intron 1 primers, SimpleChIP® Human MDM2 Intron 2 Primers #90678, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).
In humans the Myc family consists of 5 genes: c-Myc, N-Myc, L-Myc, R-Myc, and B-Myc. While c-Myc is expressed in many proliferating cells, N-Myc expression is very restricted, with highest levels during embryonic development and in the adult during B-cell development. The expression patterns and results from targeted deletion of N-Myc suggest that N-Myc plays an important role in tissue development and differentiation (5). In addition, amplification or overexpression of N-Myc has been found in human neuroblastomas and is associated with rapid progression and poor prognosis (6,7).
- Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
- Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
- Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
- Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.
- Sawai, S. et al. (1993) Development 117, 1445-1455.
- Schwab, M. et al. (1984) Proc. Natl. Acad. Sci. USA 81, 4940-4944.
- Brodeur, G.M. et al. (1984) Science 224, 1121-1124.
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- 13079 Immunohistochemistry Application Solutions Kit (Rabbit)
- 14166 Hematoxylin
- 14177 SignalStain® Mounting Medium
- 14746 SignalStain® Citrate Unmasking Solution (10X)
- 15019 Animal-Free Blocking Solution (5X)
- 4486 SimpleChIP® Human α Satellite Repeat Primers
- 5425 Normal Goat Serum
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7017 6-Tube Magnetic Separation Rack
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- 9002 SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
- 9003 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
- 9004 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
- 9005 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
- 9006 ChIP-Grade Protein G Magnetic Beads
- 9007 ChIP-Grade Protein G Agarose Beads
- 90678 SimpleChIP® Human MDM2 Intron 2 Primers
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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