Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Interleukin-28A (hIL-28A/IFN-λ2) #5164

IFN   IFN-lambda   IFN-lambda2   IL-28a  

No. Size Price
5164LC 50 µg ( With Carrier ) ¥6,860.00 现货查询 购买询价
5164LF 50 µg ( Carrier Free ) ¥6,860.00 现货查询 购买询价
5164SC 10 µg ( With Carrier ) ¥2,321.00 现货查询 购买询价
5164SF 10 µg ( Carrier Free ) ¥2,321.00 现货查询 购买询价
5164 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human IL-28A (hIL-28A) Val26-Val200 (Accession #NP_742150) was expressed in human 293 cells at Cell Signaling Technology.

CST公司生产的人重组蛋白IL-28A (hIL-28A) Val26-Val200 (Accession #NP_742150)是从人的293细胞表达而来。

Molecular Characterization

Based on amino acid sequencing, greater than 60% of recombinant hIL-28A starts at Val26 (VPVAR) and has a calculated MW of 19,754. The remainder starts at Pro27 (PVARL) or Val28 (VARLH). DTT-reduced and non-reduced protein migrate as 21 kDa polypeptides.

基于氨基酸测序的基础上,多于60%的重组hIL-28A 蛋白在Val26 (VPVAR) 起始表达,分子量为19,754Da。其余的在Pro27 (PVARL)或Val28 (VARLH)表达。 DTT还原的和未还原的蛋白作为21 kDa 多肽而迁移。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-28A. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) 重组hIL-28A通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant hIL-28A was determined in a virus protection assay. The ED50 of each lot is between 10-60 ng/ml.

hIL-28A的生物活性是通过病毒保护实验来确定的。每个批次的ED50在10-60 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hIL-28A was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-28A and staining overnight with Coomassie Blue.重组hIL-28A 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组hIL-28A 蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Bioactivity

Bioactivity

The bioactivity of hIL-28A was determined in a virus protection assay. Hep G2 cells were pre-treated with increasing concentrations of hIL-28A for 24 hours. Cells were then inoculated with encephalomyocarditis virus (EMCV) and incubated for an additional 48 hours. Surviving cells were fixed and stained with crystal violet and the OD595 was determined.hIL-28A 的生物活性是通过病毒保护试验确定的。Hep G2 经过递增浓度的hIL-28A 预处理24小时,随后细胞与闹心肌炎病毒 (EMCV) 共孵育另外的48小时。存活的细胞固定后用结晶紫染色并测定OD595数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 cells, untreated or treated with hIL-28A for 30 minutes, using Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb #9145 (upper) and Stat3 Antibody #9132 (lower).Western免疫印迹。用 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (上图)和 Stat3 Antibody #9132 (下图) 研究未经处理的和经hIL-28A 处理30分钟的Hep G2 细胞的细胞提取液。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-28A.

每微克hIL-28A内毒素含量小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-28A. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体:每微克mIFN-γ蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mIFN-γ蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

Background

IL-28A is a member of the Interferon λ family of cytokines, which includes IL-29 and IL-28B (1). IL-28A is produced by a number of cell types and shares many functions with Type I Interferons (1-3). IL-28A exhibits anti-viral activities in vitro and in vivo (1,2). IL-28A directly inhibits tumor cell proliferation and promotes anti-tumor immune responses in vivo (1,3). The IL-28 receptor is a heterodimer of the IL28-Rα and IL10-RI (1). IL-28A activates Stat1, Stat3, and Stat5 (1). IL-28Rα expression is limited to a few cell types, including plasmacytoid DC and epithelial cells (1).

IL-28B是干扰素λ细胞因子家族成员之一,包括IL-29和IL-28A(1)。IL-28B在很多类型的细胞中产生并且与参与类型I干扰素的很多功能(1-3)。IL-28B在体内和体外都存在抗病毒的活性(1,2)。很多的研究表明,IL-28B在体内直接抑制肿瘤细胞的增殖并促进抗肿瘤免疫反应(1,3)。IL-28的受体是包含IL-28Rα和 IL-10RI的异源二聚体(1)。IL-28A激活Stat1、Stat3 和Stat5 (1)。IL-28Rα的表达只是局限在一小部分的细胞类型中,包括类浆细胞DC 和上皮细胞(1)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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