Cell Signaling Technology

Product Pathways - MAPK Signaling

HSP27 (E1J4D) Rabbit mAb (IHC Preferred) #50353

No. Size Price
50353S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
50353 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 27 Rabbit IgG
IP 1:50
IHC-P 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

HSP27 (E1J4D) Rabbit mAb (IHC Preferred) recognizes endogenous levels of total HSP27 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HSP27 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HeLa (left) and HT-1080 (right) cell pellets using HSP27 (E1J4D) Rabbit mAb (IHC Preferred).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse forestomach using HSP27 (E1J4D) Rabbit mAb (IHC Preferred).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HSP27 (E1J4D) Rabbit mAb (IHC Preferred) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of HSP27 from ACHN cell extracts. Lane 1 is 10% input, lane 2 is immunoprecipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HSP27 (E1J4D) Rabbit mAb (IHC Preferred). Western blot was performed using HSP27 (E1J4D) Rabbit mAb (IHC Preferred). A conformation specific secondary antibody was used to avoid cross reactivity with IgG.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HSP27 (E1J4D) Rabbit mAb (IHC Preferred).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using HSP27 (E1J4D) Rabbit mAb (IHC Preferred).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using HSP27 (E1J4D) Rabbit mAb (IHC Preferred).

Background

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).

  1. Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
  2. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
  3. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  4. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
  5. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
  6. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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