Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

iNOS (D6B6S) Rabbit mAb (Alexa Fluor® 647 Conjugate) #48866

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Mouse, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

iNOS (D6B6S) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total iNOS protein. This antibody does not cross-react with other NOS proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1133 of mouse iNOS protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated iNOS (D6B6S) Rabbit mAb #13120.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Raw309.CR.1 cells untreated (blue) or #14011 Lipopolysaccharides (LPS) treated (green) using iNOS (D6B6S) Rabbit mAb (Alexa Fluor® 647 Conjugate).

Background

Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citruline from L-arginine, oxygen and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels (1). NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory (2,3).

  1. Tsutsui, M. (2004) J Atheroscler Thromb 11, 41-48.
  2. Son, H. et al. (1996) Cell 87, 1015-23.
  3. Hawkins, R.D. (1996) Neuron 16, 465-467.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, including use with HCS or other automated imaging applications but excluding use in combination with DNA microarrays. The buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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