Cell Signaling Technology

Product Pathways - Development

DKK1 (D5V6L) Rabbit mAb #48367

No. Size Price
48367S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
48367 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 28-40 Rabbit IgG
IP 1:100
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

DKK1 (D5V6L) Rabbit mAb recognizes endogenous levels of total DKK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DKK1 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of PC-3 (positive, left) and U-2 OS (negative, right) cells using DKK1 (D5V6L) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts of PC-3 and U-251 cells using DKK1 (D5V6L) Rabbit mAb.

Background

Dickkopf (DKK) family proteins consist of four members DKK1, DKK2, DKK3 and DKK4 that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6).

DKK1 was initially identified as an inducer of head formation in Xenopus embryos (7) and plays an important role in the regulation of bone mass (8-10). Research studies indicate that increased levels of DKK1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers (11,12), while DKK1 expression is decreased in malignant melanoma and colorectal cancers (13,14).

  1. Mao, B. et al. (2001) Nature 411, 321-5.
  2. Niehrs, C. (2006) Oncogene 25, 7469-81.
  3. Krupnik, V.E. et al. (1999) Gene 238, 301-13.
  4. Monaghan, A.P. et al. (1999) Mech Dev 87, 45-56.
  5. Mao, B. et al. (2002) Nature 417, 664-7.
  6. Davidson, G. et al. (2002) Development 129, 5587-96.
  7. Glinka, A. et al. (1998) Nature 391, 357-62.
  8. Baron, R. and Rawadi, G. (2007) Curr Osteoporos Rep 5, 73-80.
  9. MacDonald, B.T. et al. (2007) Bone 41, 331-9.
  10. Diarra, D. et al. (2007) Nat Med 13, 156-63.
  11. Forget, M.A. et al. (2007) Br J Cancer 96, 646-53.
  12. Yamabuki, T. et al. (2007) Cancer Res 67, 2517-25.
  13. Kuphal, S. et al. (2006) Oncogene 25, 5027-36.
  14. Aguilera, O. et al. (2006) Oncogene 25, 4116-21.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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