Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Filamin A (Ser2152) Antibody #4761

ABP   ABP280   alphaFLN   FLN1   FLNa   non-muscle FLN  

No. Size Price
4761S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
4761 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 280 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Filamin A (Ser2152) Antibody detects endogenous levels of filamin A only when phosphorylated at serine 2152. This antibody also reacts with filamin C when it is phosphorylated at serine 2146.

Phospho-Filamin A (Ser2152) Antibody仅检测在serine 2152位点磷酸化的内源性Filamin A蛋白。该抗体也与在serine 2146位点磷酸化的filamin C蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser2152 of human filamin A and Ser2146 of human filamin C. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源filamin A蛋白Ser2152位点和人源filamin C蛋白Ser2146位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 and HeLa cells, untreated or lamda phosphatase-treated, using Phospho-Filamin A (Ser2152) Antibody (upper) and Filamin A Antibody #4762 (lower).

使用Phospho-Filamin A (Ser2152) Antibody (上图)和Filamin A Antibody #4762 (下图),免疫印迹(Western Blot)分析HEK293和HeLa细胞中Phospho-Filamin A (Ser2152)和Filamin A蛋白水平,细胞分为未处理组和lamda phosphatase处理组。


Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (alpha-actinin domain) is located at the very amino-terminus, followed by as many as 24 tandem repeats of about 96 residues, and the dimerization domain is located at the carboxy-terminal end of th protein. In addition to actin filaments, filamins associate with other structural and signaling molecules such as beta-integrins, Rho/Rac/Cdc42, PKC, and insulin receptor, primarily through their carboxy-terminal half (1-3). Filamin A, the most abundant, and filamin B, less abundant, are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Serine 2152 of filamin A is phosphorylated by PAK1, and this phosphorylation is required for PAK1-mediated actin cytoskeleton reorganization (4).

Filamins蛋白是二聚化actin结合蛋白的家族,其功能是作为细胞粘附位点的结构性组件。对于亚细胞内信号分子的靶点它们也作为一个支架(1)。actin结合域(alpha-actinin domain)是定位在特有的氨基末端,接着是多达24串联的大约96残基重复序列,而二聚体化结构域是位于该蛋白羧基末端。除actin filaments之外,filamins与其它结构上分子和信号分子例如beta-integrins、Rho/Rac/Cdc42、PKC和insulin receptor有关联,但主要通过部分羧基末端区域(1-3)。高丰度的Filamin A蛋白和低丰度的filamin B蛋白是广泛表达的亚型,而filamin C 蛋白是在肌肉中主要表达(1)。filamin A蛋白的Serine 2152位点通过PAK1激酶磷酸化,而这个磷酸化对于PAK1介导的actin细胞骨架重组是需要的(4)。

  1. Stossel, T.P. et al. (2001) Nat Rev Mol Cell Biol 2, 138-45.
  2. Tigges, U. et al. (2003) J Biol Chem 278, 23561-9.
  3. He, H.J. et al. (2003) J Biol Chem 278, 27096-104.
  4. Vadlamudi, R.K. et al. (2002) Nat Cell Biol 4, 681-90.

Application References

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