Cell Signaling Technology

Product Pathways - Development

CELSR2 (D2M9H) XP® Rabbit mAb #47061

Cadherin   CELSR-2   CELSR2   FLAMINGO  

No. Size Price
47061S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
47061 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 320 Rabbit IgG
IHC-P 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

CELSR2 (D2M9H) XP® Rabbit mAb recognizes endogenous levels of total CELSR2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His1781 of human CELSR2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7, A549 and U-118 MG cells using CELSR2 (D2M9H) XP® Rabbit mAb (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MCF7 (left) and U-118MG (right) cell pellets using CELSR2 (D2M9H) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using CELSR2 (D2M9H) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human skin using CELSR2 (D2M9H) XP® Rabbit mAb.

Background

CELSR2 (cadherin EGF LAG seven-pass G-type receptor, also known as flamingo homolog 3 or epidermal growth factor-like protein 2) is a member of the flamingo subfamily of non-classical cadherins, part of the cadherin superfamily. CELSR2 is a 7-transmembrane helix receptor that contains nine cadherin-like domains, seven EGF-like repeats, and 2 laminin A G-type repeats (1). It shares structural characteristics of both an adhesion molecule and a G protein-coupled receptor, suggesting putatives roles in both cell-cell adhesion and juxtacrine signaling. It's function has been associated with dendrite morphogenesis (2), neural plate anterior-posterior pattern formation (3), and regulation of transcription via the Wnt signaling pathway (4). In a loss-of-function mouse model, Celsr2 deletion resulted in defects in the planar organization of ependymal cilia, leading to defective cerebrospinal fluid dynamics and hydrocephalus (5). In humans, SNPs in the CELSR2 gene cluster on chromosome 1 have been associated with enhanced risk of coronary artery disease (6).

  1. Vincent, J.B. et al. (2000) DNA Res 7, 233-5.
  2. Shima, Y. et al. (2004) Dev Cell 7, 205-16.
  3. Devenport, D. and Fuchs, E. (2008) Nat Cell Biol 10, 1257-68.
  4. Qu, Y. et al. (2014) Proc Natl Acad Sci U S A 111, E2996-3004.
  5. Tissir, F. et al. (2010) Nat Neurosci 13, 700-7.
  6. Zhou, Y.J. et al. (2015) Int J Clin Exp Pathol 8, 9543-51.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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