Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Mouse His6 Tumor Necrosis Factor-α (mHis6TNF-α) #4698

his tag   tnf-a   TNFa   TNFSF2  

No. Size Price
4698LC 50 µg ( With Carrier ) ¥6,860.00 现货查询 购买询价
4698LF 50 µg ( Carrier Free ) ¥6,860.00 现货查询 购买询价
4698SC 10 µg ( With Carrier ) ¥2,321.00 现货查询 购买询价
4698SF 10 µg ( Carrier Free ) ¥2,321.00 现货查询 购买询价
4698 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant mouse His6 TNF-α (mHis6TNF-α) Leu80-Leu235 (Accession #NP_038721) was expressed in human 293 cells at Cell Signaling Technology.

CST公司在人293细胞中生产重组鼠蛋白His6TNF-α (mHis6TNF-α) Leu80-Leu235 (Accession #NP_038721)。

Molecular Characterization

Recombinant N-terminally His6-tagged mTNF-α has a calculated MW of 20203. DTT-reduced and non-reduced protein migrate as 20 kDa polypeptides.



>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mHis6TNF-α. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) mHis6TNF-α通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。


The bioactivity of recombinant mHis6TNF-α was determined in a L-929 cell viability assay. The ED50 of each lot is between 10-40 pg/ml.

重组蛋白mHis6TNF-α的生物活性是通过L-929细胞生存能力实验确定的。每个批次的ED50在10-40 pg/ml之间。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with mHis6TNF-α for 20 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).

Coomassie Gel

Coomassie Gel

The purity of recombinant mHis6TNF-α was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mHis6TNF-α and staining overnight with Coomassie Blue.重组蛋白mHis6TNF-α的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。



The viability of L-929 cells treated with increasing concentrations of mHis6TNF-α in the presence of 2 ng/ml actinomycin D was assessed. Cells were stained with crystal violet at the end of treatment and the OD595 was determined.在2 ng/ml放射菌素D存在下 用递增浓度的重组蛋白mHis6TNF-α处理L-929 细胞并观察其生存能力的实验。处理细胞后细胞用结晶紫染色并测定OD595 数值。


Less than 0.01 ng endotoxin/1 μg mHis6TNF-α.

内毒素含量:<0.01 ng /1 μg mHis6TNF-α。


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg mHis6TNF-α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克mHis6TNF-α蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克mHis6TNF-α蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。


TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, ERK (p44/42), p38 MAPK and NF-κB) promotes the survival of cells, while TNF-α mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).

TNF-α, TNF家族的典型成员, 是II型的同源三聚体膜蛋白(1,2)。细胞膜锚定的TNF-α通过金属蛋白酶TACE/ADAM17的剪切形成可溶性的同型三聚体(2)。膜锚定和可溶性两种形式都有生物活性。TNF-α可由很多种类的细胞产生包括T细胞、B细胞、巨噬细胞和NK细胞(1)。TNF-α的细胞内响应是通过与 TNF-R1 和 TNF-R2 两个受体互作而介导的并激活细胞存活和细胞凋亡两个截然相反地过程,这两个过程主要决定于细胞的类型和生物学环境。激活激酶的通路(包括JNK, Erk (p44/42), p38 MAPK 和 NF-κB) 促使细胞的存活,TNF-α介导的对caspase-8的激活导致了程序性的细胞死亡(1,2)。TNF-α在炎症反应和宿主抵抗细菌入侵的过程特别是结核分枝杆菌发挥了重要的调控作用(3)。

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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