Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

Human BMP2 #4697

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

recombinant human BMP2 expressed in E. coli.

此重组人源BMP-2蛋白表达于E. coli。

Description

Recombinant human BMP-2 was expressed in E. coli and is supplied in a lyophilized form. A greater than 95% purity was determined by reverse-phase HPLC and SDS-PAGE.

此重组人源BMP-2蛋白在E. coli中表达,以冻干形式储存,经反相HPLC和SDS-PAGE测定纯度大于95%。

Purity

>95%

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved HT-1080 cells, untreated and treated for 30 minutes with various concentrations of Human BMP2, using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 (upper) and Smad1 Antibody #9743 (lower).使用不同浓度人BMP2处理30分钟或未处理,血清饥饿后的HT-1080细胞提取物进行Western blot实验,使用 Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 (上) and Smad1 Antibody #9743 (下)抗体检测.

Directions for Use

The working concentration of BMP-2 generally ranges from 20-50 ng/ml.

BMP-2工作浓度约20-50ng/ml。

Background

Bone morphogenetic proteins (BMPs) were first identified as molecules that can induce ectopic bone and cartilage formation (1,2). BMPs belongs to the TGF-β superfamily, playing many diverse functions during development (3). BMPs are synthesized as precursor proteins and then processed by cleavage to release the c-terminal mature BMP. BMPs initiate signaling by binding to a receptor complex containing type I and type II serine/threonine receptor kinases that then phosphorylate Smad (mainly Smad1, 5 and 8), resulting the translocation of Smad into the nucleus. BMP was also reported to activate MAPK pathways in some systems (3,4).

骨形态发生蛋白首次被确定为诱导异位骨和软骨形成的分子[1,2]。BMP蛋白属于TGF- β超家族,在发育中起多种功能[3]。BMP蛋白先被合成为前体蛋白,然后被加工剪切释放C末端的成熟体BMP蛋白。BMP蛋白通过和受体复合物结合起始信号通路,这些受体复合物包含I 型和 II型丝氨酸/苏氨酸受体激酶,激酶可磷酸化Smad(主要是Smad1,5,8)导致Smad转移进入核内。BMP蛋白被报道在某些系统中激活MAPK通路[3,4]。

  1. Wang, E.A. et al. (1988) Proc Natl Acad Sci USA 85, 9484-8.
  2. Wozney, J.M. et al. (1988) Science 242, 1528-34.
  3. Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61.
  4. Nohe, A. et al. (2004) Cell Signal 16, 291-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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