Cell Signaling Technology

Product Pathways - Metabolism

NRF1 (D9K6P) Rabbit mAb #46743

Alpha palindromic-binding protein   Alpha-pal   NRF-1   NRF1   Nuclear respiratory factor 1  

No. Size Price
46743S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
46743 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 68 Rabbit IgG
IP 1:50
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

NRF1 (D9K6P) Rabbit mAb recognizes endogenous levels of total NRF1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala175 of human NRF1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NRF1 (D9K6P) Rabbit mAb.



Immunoprecipitation of NRF1 from HepG2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is NRF1 (D9K6P) Rabbit mAb. Western blot analysis was performed using NRF1 (D9K6P) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells and either 5 µl of NRF1 (D9K6P) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human MEF2A Exon 1 Primers #26727, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Nuclear respiratory factor 1 (NRF1) was identified as a transcription activator for the gene encoding cytochrome c (1). It was later found to play a role in the nuclear control of mitochondrial function (1). PGC-1 induces the expression of NRF1 and NRF2 (2). NRF1, along with the coactivator PGC-1, stimulates the promoter of mitochondrial transcription factor A, which regulates mitochondrial biogenensis and function (2).

  1. Virbasius, C.A. et al. (1993) Genes Dev 7, 2431-45.
  2. Wu, Z. et al. (1999) Cell 98, 115-24.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :