Cell Signaling Technology

Product Pathways - Transcription Factors

TWIST1 Antibody #46702

No. Size Price
46702S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
46702 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 26 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

TWIST1 Antibody detects endogenous levels of total TWIST1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TWIST1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extract from SH-SY5Y cells using TWIST1 Antibody.

Background

TWIST1 is a basic helix-loop-helix (b-HLH) transcription factor that functions as a master regulator of embryonic morphogenesis and plays essential roles in mesenchymal differentiation and osteogenic determination (1-3). Mutations affecting the b-HLH domain of the TWIST1 gene have been associated with Saethre-Chotzen syndrome, an autosomal dominant craniosynostosis disorder causing craniofacial and limb abnormalities (4,5). TWIST1 is upregulated in various human tumors and may play a role in EMT (epithelial-mesenchymal transition) and metastasis (6,7). Upregulation of TWIST1 may contribute to resistance to Taxol and microtubule regulating drugs in tumors (8).

  1. Thisse, B. et al. (1987) Nucleic Acids Res. 15, 3439-3453.
  2. Lee, M.S. et al. (1999) J. Cell Biochem. 75, 566-577.
  3. Chen, Z.F. and Behringer, R.R. (1995) Genes Dev. 9, 686-699.
  4. Howard, T.D. et al. (1997) Nat. Genet. 15, 36-41.
  5. Connerney, J. et al. (2006) Dev. Dyn. 235, 1345-1357.
  6. Watanabe, O. et al. (2004) Anticancer Res. 24, 3851-3856.
  7. Yang, J. et al. (2004) Cell 117, 927-939.
  8. Wang, X. et al. (2004) Oncogene 23, 474-482.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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