Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

IL-2Rβ (D4X3H) Rabbit mAb #46307

No. Size Price
46307S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
46307 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 79 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

IL-2Rβ (D4X3H) Rabbit mAb recognizes endogenous levels of total IL-2Rβ protein. This antibody cross-reacts with proteins of unknown identity at 38-42 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu428 of human IL-2Rβ protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IL-2Rβ (D4X3H) Rabbit mAb (upper), and β-Actin (D6A8) Rabbit mAb #8457 (lower).



Immunoprecipitation of IL-2Rβ protein from HDLM-2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IL-2Rβ (D4X3H) Rabbit mAb. Western blot analysis was performed using IL-2Rβ (D4X3H) Rabbit mAb.


Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).

IL-2Rβ, also known as CD122, is a type I transmembrane protein that is expressed by natural killer cells and certain subsets of T cells in the periphery (6). IL-2Rβ surface expression is upregulated following antigen-induced activation. A heteromeric complex composed of IL-2Rβ and γc forms the intermediate affinity IL-2 receptor whereas a complex comprised of all three chains forms the high affinity receptor. Signaling through IL-2Rβ is mediated by Jak1 (7), which phosphorylates numerous tyrosine residues in the cytoplasmic tail to promote the recruitment of the adaptor Shc to IL-2Rβ and enhance IL-2 receptor signaling (8, 9).

  1. Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79.
  2. Gaffen, S.L. and Liu, K.D. (2004) Cytokine 28, 109-23.
  3. Fehérvari, Z. et al. (2006) Trends Immunol 27, 109-11.
  4. Antony, P.A. et al. (2006) J Immunol 176, 5255-66.
  5. Jaleco, S. et al. (2003) J Immunol 171, 61-8.
  6. Shevach, E.M. (2012) Trends Immunol 33, 626-32.
  7. Nelson, B.H. and Willerford, D.M. (1998) Adv Immunol 70, 1-81.
  8. Gaffen, S.L. (2001) Cytokine 14, 63-77.
  9. Yu, A. et al. (2009) Immunity 30, 204-17.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :