Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Doublecortin (Ser297) Antibody #4605

DBCN   DCX   doublin   Lis-X   Lissencephalin-X   LISX   Neuronal migration protein doublecortin  

No. Size Price
4605S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
4605 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat, Endogenous 45 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Phospho-Doublecortin (Ser297) Antibody detects endogenous levels of doublecortin only when phosphorylated at Ser297.

Phospho-Doublecortin (Ser297) Antibody 兔多抗识别内源性的Ser297磷酸化的doublecortin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser297 of doublecortin. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from fetal rat and fetal human brain using Phospho-Doublecortin (Ser297) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer.

Western blot分析胎大鼠和人胚胎大脑提取物,使用的抗体是Phospho-Doublecortin (Ser297) Antibody 兔多抗。抗体的磷酸化特异性由western转膜后对膜进行(+)或不进行(-)小牛肠碱性磷酸酶(CIP)处理验证。


Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).


  1. Gleeson, J.G. et al. (1998) Cell 92, 63-72.
  2. Reiner, O. et al. (2004) Cell Cycle 3, 747-51.
  3. Gdalyahu, A. et al. (2004) EMBO J 23, 823-32.
  4. Schaar, B.T. et al. (2004) Neuron 41, 203-13.
  5. Tanaka, T. et al. (2004) Neuron 41, 215-27.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :